Metabolism of 1,25-dihydroxyvitamin D3: Evidence for side-chain oxidation

Rajiv Kumar, Deborah Harnden, Hector F. DeLuca

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Approximately 7% of a 650-pmol dose of 25-hydroxy[26,27-14C]vitamin D3 and 25% of a 325-pmol dose of 1,25-dihydroxy[26,27-14C]vitamin D3 are metabolized to 14CO2 by vitamin D deficient rats. Nephrectomy prevents the metabolism of 25-hydroxy[26,27-14C]vitamin D3 to 14CO2 but not that of 1,25-dihydroxy[26,27-14C]vitamin D3. Less than 5% of the 14C from 24,25-dihydroxy[26,27-14C]vitamin D3 is metabolized to 14CO2. Feeding diets high in calcium and supplemented with vitamin D3 markedly diminishes the amount of 14CO2 formed from 25-hydroxy[26,27-14C]vitamin D3 but not that from 1,25-dihydroxy[26,27-14C]vitamin D3. These results provide strong evidence that only 1-hydroxylated vitamin D compounds and especially 1,25-dihydroxyvitamin D3 undergo side-chain oxidation and cleavage to yield an unknown metabolite and CO2.

Original languageEnglish (US)
Pages (from-to)2420-2423
Number of pages4
JournalBiochemistry
Volume15
Issue number11
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Metabolism of 1,25-dihydroxyvitamin D<sub>3</sub>: Evidence for side-chain oxidation'. Together they form a unique fingerprint.

Cite this