Metabolism of 1,25-Dihydroxyvitamin D3: Evidence for Side-Chain Oxidation

Rajiv Kumar; Deborah Harnden; Hector F. DeLuca

doi:10.1021/bi00656a027

Metabolism of 1,25-Dihydroxyvitamin D³: Evidence for Side-Chain Oxidation

Rajiv Kumar, Deborah Harnden, Hector F. DeLuca

Nephrology and Hypertension

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Abstract

Approximately 7% of a 650-pmol dose of 25- hydroxy [26,27-¹⁴C] vitamin D₃ and 25% of a 325-pmol dose of 1,25-dihydroxy[26,27-¹⁴C]vitamin D₃ are metabolized to ¹⁴CO₂ by vitamin D deficient rats. Nephrectomy prevents the metabolism of 25-hydroxy [26,27-¹⁴C] vitamin D₃ to ¹⁴CO₂ but not that of 1,25-dihydroxy [26,27-¹⁴C] vitamin D₃. Less than 5% of the¹⁴C from 24,25-dihydroxy[26,27-¹⁴C]vitamin D₃ is metabolized to¹⁴CO₂. Feeding diets high in calcium and supplemented with vitamin D₃ markedly diminishes the amount of¹⁴CO₂ formed from 25-hydroxy[26,27-¹⁴C]vitamin D₃ but not that from 1,25-dihydroxy[26,27-¹⁴C]vitamin D₃. These results provide strong evidence that only 1-hydroxylated vitamin D compounds and especially 1,25-dihydroxyvitamin D₃ undergo side-chain oxidation and cleavage to yield an unknown metabolite and CO₂.

Original language	English (US)
Pages (from-to)	2420-2423
Number of pages	4
Journal	Biochemistry
Volume	15
Issue number	11
DOIs	https://doi.org/10.1021/bi00656a027
State	Published - Jun 1 1976

ASJC Scopus subject areas

Biochemistry

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title = "Metabolism of 1,25-Dihydroxyvitamin D3: Evidence for Side-Chain Oxidation",

abstract = "Approximately 7% of a 650-pmol dose of 25- hydroxy [26,27-14C] vitamin D3 and 25% of a 325-pmol dose of 1,25-dihydroxy[26,27-14C]vitamin D3 are metabolized to 14CO2 by vitamin D deficient rats. Nephrectomy prevents the metabolism of 25-hydroxy [26,27-14C] vitamin D3 to 14CO2 but not that of 1,25-dihydroxy [26,27-14C] vitamin D3. Less than 5% of the14C from 24,25-dihydroxy[26,27-14C]vitamin D3 is metabolized to14CO2. Feeding diets high in calcium and supplemented with vitamin D3 markedly diminishes the amount of14CO2 formed from 25-hydroxy[26,27-14C]vitamin D3 but not that from 1,25-dihydroxy[26,27-14C]vitamin D3. These results provide strong evidence that only 1-hydroxylated vitamin D compounds and especially 1,25-dihydroxyvitamin D3 undergo side-chain oxidation and cleavage to yield an unknown metabolite and CO2.",

author = "Rajiv Kumar and Deborah Harnden and DeLuca, {Hector F.}",

year = "1976",

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T1 - Metabolism of 1,25-Dihydroxyvitamin D3

T2 - Evidence for Side-Chain Oxidation

AU - Kumar, Rajiv

AU - Harnden, Deborah

AU - DeLuca, Hector F.

PY - 1976/6/1

Y1 - 1976/6/1

N2 - Approximately 7% of a 650-pmol dose of 25- hydroxy [26,27-14C] vitamin D3 and 25% of a 325-pmol dose of 1,25-dihydroxy[26,27-14C]vitamin D3 are metabolized to 14CO2 by vitamin D deficient rats. Nephrectomy prevents the metabolism of 25-hydroxy [26,27-14C] vitamin D3 to 14CO2 but not that of 1,25-dihydroxy [26,27-14C] vitamin D3. Less than 5% of the14C from 24,25-dihydroxy[26,27-14C]vitamin D3 is metabolized to14CO2. Feeding diets high in calcium and supplemented with vitamin D3 markedly diminishes the amount of14CO2 formed from 25-hydroxy[26,27-14C]vitamin D3 but not that from 1,25-dihydroxy[26,27-14C]vitamin D3. These results provide strong evidence that only 1-hydroxylated vitamin D compounds and especially 1,25-dihydroxyvitamin D3 undergo side-chain oxidation and cleavage to yield an unknown metabolite and CO2.

AB - Approximately 7% of a 650-pmol dose of 25- hydroxy [26,27-14C] vitamin D3 and 25% of a 325-pmol dose of 1,25-dihydroxy[26,27-14C]vitamin D3 are metabolized to 14CO2 by vitamin D deficient rats. Nephrectomy prevents the metabolism of 25-hydroxy [26,27-14C] vitamin D3 to 14CO2 but not that of 1,25-dihydroxy [26,27-14C] vitamin D3. Less than 5% of the14C from 24,25-dihydroxy[26,27-14C]vitamin D3 is metabolized to14CO2. Feeding diets high in calcium and supplemented with vitamin D3 markedly diminishes the amount of14CO2 formed from 25-hydroxy[26,27-14C]vitamin D3 but not that from 1,25-dihydroxy[26,27-14C]vitamin D3. These results provide strong evidence that only 1-hydroxylated vitamin D compounds and especially 1,25-dihydroxyvitamin D3 undergo side-chain oxidation and cleavage to yield an unknown metabolite and CO2.

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Metabolism of 1,25-Dihydroxyvitamin D³: Evidence for Side-Chain Oxidation

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