Membrane marker characterization of the eosinophil colony-forming cell

J. H. Butterfield, A. B. Eisenbrey, G. J. Gleich

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the membrane markers of the eosinophil colony-forming cell (EO-CFC). Eosinophil colonies were identified by their ability to show densely packed cell growth and by staining for cyanide-resistant peroxidase activity. Bone marrow cells were characterized prior to soft agar culture by one of two methods: (1) standard erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosetting, or (2) by the human T- and B-cell surface markers, Leu 1 and HLA-DR, using the fluorescence-activated cell sorter. Eosinophil colonies developed mainly from the nonrosetting, null cell population. EO-CFC lacked the human T-cell marker Leu 1, but demonstrated the human B-cell marker, HLA-DR. EO-CFC were also present in peripheral blood and possessed similar properties to those in the bone marrow. The results indicate that EO-CFC are derived from the null lymphocyte cell population and possess HLA-DR markers. Cells forming eosinophil colonies (EO-CFC) are present in normal bone marrow and differ in many respects from the granulocyte-monocyte-forming cell (GM-CFC) (Dao et al., 1977a,b; Inoue & Ottenbreit, 1978). Although GM-CFC are greatly concentrated in the null cell subset of peripheral blood lymphocytes (Richman et al., 1978), it is not known whether EO-CFC are also null cells. We have investigated EO-CFC in normal human bone marrow and peripheral blood, utilizing cell segregation prior to soft agar culture to specifically characterize EO-CFC surface markers and growth characteristics. Our results indicate that the EO-CFC is concentrated in the null cell lymphocyte subset and bears the HLA-DR(+) marker.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalBritish Journal of Haematology
Volume51
Issue number2
StatePublished - 1982

Fingerprint

Eosinophils
Null Lymphocytes
Membranes
HLA-DR Antigens
Bone Marrow
Agar
B-Lymphocytes
Erythrocytes
Cell Separation
Lymphocyte Subsets
Cyanides
Growth
Granulocytes
Bone Marrow Cells
Population
Peroxidase
Monocytes
Blood Cells
Fluorescence
Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Butterfield, J. H., Eisenbrey, A. B., & Gleich, G. J. (1982). Membrane marker characterization of the eosinophil colony-forming cell. British Journal of Haematology, 51(2), 209-216.

Membrane marker characterization of the eosinophil colony-forming cell. / Butterfield, J. H.; Eisenbrey, A. B.; Gleich, G. J.

In: British Journal of Haematology, Vol. 51, No. 2, 1982, p. 209-216.

Research output: Contribution to journalArticle

Butterfield, JH, Eisenbrey, AB & Gleich, GJ 1982, 'Membrane marker characterization of the eosinophil colony-forming cell', British Journal of Haematology, vol. 51, no. 2, pp. 209-216.
Butterfield, J. H. ; Eisenbrey, A. B. ; Gleich, G. J. / Membrane marker characterization of the eosinophil colony-forming cell. In: British Journal of Haematology. 1982 ; Vol. 51, No. 2. pp. 209-216.
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