Mechanism by which caloric restriction improves insulin sensitivity in sedentary obese adults

Matthew L. Johnson, Klaus Distelmaier, Ian R Lanza, Brian A. Irving, Matthew M. Robinson, Adam R. Konopka, Gerald I. Shulman, K Sreekumaran Nair

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Caloric restriction (CR) improves insulin sensitivity and reduces the incidence of diabetes in obese individuals. The underlying mechanisms whereby CR improves insulin sensitivity are not clear. We evaluated the effect of 16 weeks of CR on whole-body insulin sensitivity by pancreatic clamp before and after CR in 11 obese participants (BMI = 35 kg/m2) compared with 9 matched control subjects (BMI = 34 kg/m2). Compared with the control subjects, CR increased the glucose infusion rate needed to maintain euglycemia during hyperinsulinemia, indicating enhancement of peripheral insulin sensitivity. This improvement in insulin sensitivity was not accompanied by changes in skeletal muscle mitochondrial oxidative capacity or oxidant emissions, nor were there changes in skeletal muscle ceramide, diacylglycerol, or amino acid metabolite levels. However, CR lowered insulin-stimulated thioredoxin-interacting protein (TXNIP) levels and enhanced nonoxidative glucose disposal. These results support a role for TXNIP in mediating the improvement in peripheral insulin sensitivity after CR.

Original languageEnglish (US)
Pages (from-to)74-84
Number of pages11
JournalDiabetes
Volume65
Issue number1
DOIs
StatePublished - Jan 1 2016

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Caloric Restriction
Insulin Resistance
Thioredoxins
Skeletal Muscle
Glucose
Ceramides
Diglycerides
Hyperinsulinism
Oxidants
Proteins
Insulin
Amino Acids
Incidence

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Mechanism by which caloric restriction improves insulin sensitivity in sedentary obese adults. / Johnson, Matthew L.; Distelmaier, Klaus; Lanza, Ian R; Irving, Brian A.; Robinson, Matthew M.; Konopka, Adam R.; Shulman, Gerald I.; Nair, K Sreekumaran.

In: Diabetes, Vol. 65, No. 1, 01.01.2016, p. 74-84.

Research output: Contribution to journalArticle

Johnson, ML, Distelmaier, K, Lanza, IR, Irving, BA, Robinson, MM, Konopka, AR, Shulman, GI & Nair, KS 2016, 'Mechanism by which caloric restriction improves insulin sensitivity in sedentary obese adults', Diabetes, vol. 65, no. 1, pp. 74-84. https://doi.org/10.2337/db15-0675
Johnson ML, Distelmaier K, Lanza IR, Irving BA, Robinson MM, Konopka AR et al. Mechanism by which caloric restriction improves insulin sensitivity in sedentary obese adults. Diabetes. 2016 Jan 1;65(1):74-84. https://doi.org/10.2337/db15-0675
Johnson, Matthew L. ; Distelmaier, Klaus ; Lanza, Ian R ; Irving, Brian A. ; Robinson, Matthew M. ; Konopka, Adam R. ; Shulman, Gerald I. ; Nair, K Sreekumaran. / Mechanism by which caloric restriction improves insulin sensitivity in sedentary obese adults. In: Diabetes. 2016 ; Vol. 65, No. 1. pp. 74-84.
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