TY - JOUR
T1 - Measurement of the susceptibility of paramagnetically labeled cells with paramagnetic solutions
AU - Russell, Anthony P.
AU - Evans, Christopher H.
AU - Westcott, Vernon C.
N1 - Funding Information:
The original investigation of the “isomagnetic” method was made at the Foxboro Company. Their sup port is gratefully acknowledged. This work was performed at Boston University Medical Center and at the University of Pittsburgh. The work at the University of Pittsburgh was supported by Grant PCM 8219930 from the National Science Foundation. The work at Boston University was made possible through the kindness of Professor Judith Marquis of the Department of Pharmacology, Boston University Medical Center, who provided laboratory support, in part under a grant from the U.S. Army Research Office (DAAG29-85-K-007 1). Drs. D. Kelland and M. Takayasu of the Francis Bitter National Magnet Laboratory of the Massachusetts Institute of Technology and M. Graham of Coulter Biomedical Research Corporation made valuable contributions to this paper in the form of advice, criticism, and discussion of the equations. Their interest is greatly appreciated.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987/7
Y1 - 1987/7
N2 - A method of measuring the volumetric magnetic susceptibility, in which magnetically labeled cells or other particles are suspended in a paramagnetic solution of known susceptibility over the poles of a magnet, is presented. If the cells are more magnetic than the solution, they are attracted toward the poles; if they are less magnetic, they are repelled. If they have the same susceptibility as the solution, they do not move. Under this condition, the cells are said to be "isomagnetic" with the surrounding solution. Since the volumetric susceptibility of this solution is known, the susceptibility of the cells is obtained. Using the "isomagnetic" method, the volumetric susceptibilities of test metal powders were determined within ±8 × 10-6 SI units. Yeast, colonic carcinoma, and liver cells, rendered magnetic with erbium chloride, had susceptibilities ranging from 13 to 20 × 10-6. Particles of articular cartilage treated with erbium chloride were heterogeneous, with susceptibilities ranging between 50 and 125 × 10-6, while particles of bone had a susceptibility of 560 to 580 × 10-6. Eukaryotic cells labeled with ferritin attained susceptibilities of less than 1 × 10-6.
AB - A method of measuring the volumetric magnetic susceptibility, in which magnetically labeled cells or other particles are suspended in a paramagnetic solution of known susceptibility over the poles of a magnet, is presented. If the cells are more magnetic than the solution, they are attracted toward the poles; if they are less magnetic, they are repelled. If they have the same susceptibility as the solution, they do not move. Under this condition, the cells are said to be "isomagnetic" with the surrounding solution. Since the volumetric susceptibility of this solution is known, the susceptibility of the cells is obtained. Using the "isomagnetic" method, the volumetric susceptibilities of test metal powders were determined within ±8 × 10-6 SI units. Yeast, colonic carcinoma, and liver cells, rendered magnetic with erbium chloride, had susceptibilities ranging from 13 to 20 × 10-6. Particles of articular cartilage treated with erbium chloride were heterogeneous, with susceptibilities ranging between 50 and 125 × 10-6, while particles of bone had a susceptibility of 560 to 580 × 10-6. Eukaryotic cells labeled with ferritin attained susceptibilities of less than 1 × 10-6.
KW - cell susceptibility
KW - erbium
KW - ferritin
KW - lanthanides
KW - magnetic separation
KW - magnetic susceptibility
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U2 - 10.1016/0003-2697(87)90384-8
DO - 10.1016/0003-2697(87)90384-8
M3 - Article
C2 - 3314577
AN - SCOPUS:0023374463
SN - 0003-2697
VL - 164
SP - 181
EP - 189
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -