Measles virus expressed Helicobacter pylori neutrophil-activating protein significantly enhances the immunogenicity of poor immunogens

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Helicobacter pylori neutrophil-activating protein (NAP) is a toll-like receptor 2 (TLR2) agonist and potent immunomodulator inducing Th1-type immune response. Here we present data about characterization of the humoral immune response against NAP-tagged antigens, encoded by attenuated measles virus (MV) vector platform, in MV infection susceptible type I interferon receptor knockout and human CD46 transgenic (Ifnarko-CD46Ge) mice. Immunogenicity of MV expressing a full-length human immunoglobulin lambda light chain (MV-lambda) was compared to that of MV expressing lambda-NAP chimeric protein (MV-lambda-NAP). MV-lambda-NAP immunized Ifnarko-CD46Ge mice developed significantly higher (6-20-fold) anti-lambda ELISA titers as compared to the MV-lambda-immunized control animal group, indicating that covalently-linked NAP co-expression significantly enhanced lambda immunogenicity. In contrast, ELISA titers against MV antigens were not significantly different between the animals vaccinated with MV-lambda or MV-lambda-NAP. NAP-tagged antigen expression did not affect development of protective anti-measles immunity. Both MV-lambda and MV-lambda-NAP-immunized groups showed strong virus neutralization serum titers in plaque reduction microneutralization test. These results demonstrated that MV-encoded lambda-NAP is highly immunogenic as compared to the unmodified full-length lambda chain. Boost of immune response to poor immunogens using live vectors expressing NAP-tagged chimeric antigens is an attractive approach with potential application in immunoprophylaxis of infectious diseases and cancer immunotherapy.

Original languageEnglish (US)
Pages (from-to)4795-4801
Number of pages7
JournalVaccine
Volume31
Issue number42
DOIs
StatePublished - Oct 1 2013

Fingerprint

Measles virus
Helicobacter pylori
neutrophils
immune response
antigens
Neutrophils
proteins
Proteins
Antigens
Helicobacter pylori neutrophil-activating protein A
Immunoglobulin lambda-Chains
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
recombinant fusion proteins
Toll-Like Receptor 2
immunomodulators
immunotherapy
mice
Measles
Immunologic Factors

Keywords

  • Chimeric antigens
  • Helicobacter pylori
  • Immunogenicity
  • Measles virus vaccine
  • Neutrophil-activating protein

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Measles virus expressed Helicobacter pylori neutrophil-activating protein significantly enhances the immunogenicity of poor immunogens. / Iankov, Ianko D.; Federspiel, Mark J; Galanis, Evanthia.

In: Vaccine, Vol. 31, No. 42, 01.10.2013, p. 4795-4801.

Research output: Contribution to journalArticle

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