TY - JOUR
T1 - Mast cells control the expansion and differentiation of IL-10-competent B cells
AU - Mion, Francesca
AU - D'Incà, Federica
AU - Danelli, Luca
AU - Toffoletto, Barbara
AU - Guarnotta, Carla
AU - Frossi, Barbara
AU - Burocchi, Alessia
AU - Rigoni, Alice
AU - Gerdes, Norbert
AU - Lutgens, Esther
AU - Tripodo, Claudio
AU - Colombo, Mario P.
AU - Rivera, Juan
AU - Vitale, Gaetano
AU - Pucillo, Carlo E.
N1 - Publisher Copyright:
Copyright © 2014 by The American Association of Immunologists, Inc. All rights reserved.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - The discovery of B cell subsets with regulatory properties, dependent on IL-10 production, has expanded our view on the mechanisms that control inflammation. Regulatory B cells acquire the ability to produce IL-10 in a stepwise process: first, they become IL-10 competent, a poised state in which B cells are sensitive to trigger signals but do not actually express the Il-10 gene; then, when exposed to appropriate stimuli, they start producing IL-10. Even if the existence of IL-10-competent B cells is now well established, it is not yet known how different immune cell types cross talk with B cells and affect IL-10-competent B cell differentiation and expansion. Mast cells (MCs) contribute to the differentiation and influence the effector functions of various immune cells, including B lymphocytes. In this study, we explored whether MCs could play a role in the expansion of IL-10-competent B cells and addressed the in vivo relevance of MC deficiency on the generation of these cells. We show that MCs can expand IL-10-competent B cells, but they do not directly induce IL-10 production; moreover, the absence of MCs negatively affects IL-10-competent B cell differentiation. Noteworthy, our findings reveal that the CD40L/CD40 axis plays a significant role in MC-driven expansion of IL-10-competent B cells in vitro and highlight the importance of MC CD40L signaling in the colon.
AB - The discovery of B cell subsets with regulatory properties, dependent on IL-10 production, has expanded our view on the mechanisms that control inflammation. Regulatory B cells acquire the ability to produce IL-10 in a stepwise process: first, they become IL-10 competent, a poised state in which B cells are sensitive to trigger signals but do not actually express the Il-10 gene; then, when exposed to appropriate stimuli, they start producing IL-10. Even if the existence of IL-10-competent B cells is now well established, it is not yet known how different immune cell types cross talk with B cells and affect IL-10-competent B cell differentiation and expansion. Mast cells (MCs) contribute to the differentiation and influence the effector functions of various immune cells, including B lymphocytes. In this study, we explored whether MCs could play a role in the expansion of IL-10-competent B cells and addressed the in vivo relevance of MC deficiency on the generation of these cells. We show that MCs can expand IL-10-competent B cells, but they do not directly induce IL-10 production; moreover, the absence of MCs negatively affects IL-10-competent B cell differentiation. Noteworthy, our findings reveal that the CD40L/CD40 axis plays a significant role in MC-driven expansion of IL-10-competent B cells in vitro and highlight the importance of MC CD40L signaling in the colon.
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U2 - 10.4049/jimmunol.1302593
DO - 10.4049/jimmunol.1302593
M3 - Article
C2 - 25267976
AN - SCOPUS:84908147304
SN - 0022-1767
VL - 193
SP - 4568
EP - 4579
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -