MALDI-TOF MS for the diagnosis of infectious diseases

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Background: First introduced into clinical microbiology laboratories in Europe, MALDI-TOF MS is being rapidly embraced by laboratories around the globe. Although it has multiple applications, its widespread adoption in clinical microbiology relates to its use as an inexpensive, easy, fast, and accurate method for identification of grown bacteria and fungi based on automated analysis of the mass distribution of bacterial proteins.

Content: This review provides a historical perspective on this new technology. Modern applications in the clinical microbiology laboratory are reviewed with a focus on the most recent publications in the field. Identification of aerobic and anaerobic bacteria, mycobacteria, and fungi are discussed, as are applications for testing urine and positive blood culture bottles. The strengths and limitations of MALDI-TOF MS applications in clinical microbiology are also addressed.

Summary: MALDI-TOFMSis a tool for rapid, accurate, and cost-effective identification of cultured bacteria and fungi in clinical microbiology. The technology is automated, high throughput, and applicable to a broad range of common as well as esoteric bacteria and fungi. MALDI-TOF MS is an incontrovertibly beneficial technology for the clinical microbiology laboratory.

Original languageEnglish (US)
Pages (from-to)100-111
Number of pages12
JournalClinical Chemistry
Volume61
Issue number1
DOIs
StatePublished - Jan 1 2015

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Microbiology
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Communicable Diseases
Fungi
Bacteria
Technology
Aerobic Bacteria
Bacterial Proteins
Anaerobic Bacteria
Bottles
Mycobacterium
Blood
Throughput
Urine
Costs and Cost Analysis
Testing
Costs

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Medicine(all)

Cite this

MALDI-TOF MS for the diagnosis of infectious diseases. / Patel, Robin.

In: Clinical Chemistry, Vol. 61, No. 1, 01.01.2015, p. 100-111.

Research output: Contribution to journalArticle

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