TY - JOUR
T1 - Magnesium plus mexiletine inhibit energy usage and protect retinas against ischemia
AU - Maynard, Kenneth I.
AU - Quiñones-Hinojosa, Alfredo
AU - Ogilvy, Christopher S.
PY - 1998/12/21
Y1 - 1998/12/21
N2 - We determined whether exogenous Mg2+ and/or mexiletine (Mex), which are reported to be neuroprotective agents, reduced neuronal energy requirements and protected against ischemia, using isolated rabbit retinas. Under non-ischemic conditions, Mex (300 μM) and the combination of Mg2+ (1 mM) plus Mex (300 μM) significantly reduced glucose utilization, by 19% and 31%, respectively. The combination of Mg2+ plus Mex, but not either agent alone, significantly reduced lactate production (by 18%; p < 0.05). When added during 2 h of ischemia (simulated by the reduction of oxygen from 95% to 15% and of glucose from 6 mM to 1 mM), Mg2+ plus Mex improved the recovery of glucose utilization (p < 0.01), lactate production (p < 0.05) and neuronal function (p < 0.05) for 3 h following return to control (post- ischemia/recovery) conditions. Thus reducing energy demands by blocking functions during temporary ischemia, protects neurons from irreversible functional damage.
AB - We determined whether exogenous Mg2+ and/or mexiletine (Mex), which are reported to be neuroprotective agents, reduced neuronal energy requirements and protected against ischemia, using isolated rabbit retinas. Under non-ischemic conditions, Mex (300 μM) and the combination of Mg2+ (1 mM) plus Mex (300 μM) significantly reduced glucose utilization, by 19% and 31%, respectively. The combination of Mg2+ plus Mex, but not either agent alone, significantly reduced lactate production (by 18%; p < 0.05). When added during 2 h of ischemia (simulated by the reduction of oxygen from 95% to 15% and of glucose from 6 mM to 1 mM), Mg2+ plus Mex improved the recovery of glucose utilization (p < 0.01), lactate production (p < 0.05) and neuronal function (p < 0.05) for 3 h following return to control (post- ischemia/recovery) conditions. Thus reducing energy demands by blocking functions during temporary ischemia, protects neurons from irreversible functional damage.
KW - Energy metabolism
KW - Ischemia
KW - Metabolic inhibition
KW - Neuroprotection
KW - Stroke
UR - http://www.scopus.com/inward/record.url?scp=0345471357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345471357&partnerID=8YFLogxK
U2 - 10.1097/00001756-199812210-00026
DO - 10.1097/00001756-199812210-00026
M3 - Article
C2 - 9926863
AN - SCOPUS:0345471357
SN - 0959-4965
VL - 9
SP - 4141
EP - 4144
JO - NeuroReport
JF - NeuroReport
IS - 18
ER -