Macrophages, myofibroblasts and neointimal hyperplasia after coronary artery injury and repair

Antoni Bayes-Genis, Julie H. Campbell, P. J. Carlson, David Holmes, Robert S. Schwartz

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Macrophages participate in the restenosis process through the release of cytokines, metalloproteinases and growth factors. Studies of peritoneal granulation tissue suggest that macrophages may be precursors of myofibroblasts. This study examined the contribution of monocyte/macrophage lineage cells to neointimal cellular mass in a porcine model of thermal vascular injury. Thermal coronary artery injury caused medial smooth muscle cell necrosis and transformation of the media into an extracellular matrix barrier. The neointimal hyperplasia that developed over the injury sites was evaluated by light microscopy, electron microscopy and immunohistochemistry. At day 3, blood monocytes were adhered to the vessel wall and infiltrated the fibrotic media. At day 14, 42±3.9% of neointimal cells had a monocytic nuclear morphology and expressed macrophage-specific antigen SWC3 (identified by monoclonal antibody DH59B). Moreover, 9.2±1.8% of neointimal cells co-expressed SWC3 and α-smooth muscle actin and had ultrastructural characteristics intermediate between macrophages and myofibroblasts. At day 28, 10.5±3.5% of cells expressed SWC3 and 5.2±1.8% of cells co-expressed SWC3 and α-smooth muscle actin. This study indicates that hematopoietic cells of monocyte/macrophage lineage abundantly populate the neointima in the process of lesion formation and may be precursors of neointimal myofibroblasts after thermal vascular injury.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalAtherosclerosis
Volume163
Issue number1
DOIs
StatePublished - Jun 20 2002

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Myofibroblasts
Hyperplasia
Coronary Vessels
Macrophages
Wounds and Injuries
Monocytes
Hot Temperature
Vascular System Injuries
Smooth Muscle
Actins
Neointima
Granulation Tissue
Metalloproteases
Smooth Muscle Myocytes
Extracellular Matrix
Microscopy
Intercellular Signaling Peptides and Proteins
Electron Microscopy
Necrosis
Swine

Keywords

  • Macrophages
  • Myofibroblasts
  • Neointimal hyperplasia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Macrophages, myofibroblasts and neointimal hyperplasia after coronary artery injury and repair. / Bayes-Genis, Antoni; Campbell, Julie H.; Carlson, P. J.; Holmes, David; Schwartz, Robert S.

In: Atherosclerosis, Vol. 163, No. 1, 20.06.2002, p. 89-98.

Research output: Contribution to journalArticle

Bayes-Genis, Antoni ; Campbell, Julie H. ; Carlson, P. J. ; Holmes, David ; Schwartz, Robert S. / Macrophages, myofibroblasts and neointimal hyperplasia after coronary artery injury and repair. In: Atherosclerosis. 2002 ; Vol. 163, No. 1. pp. 89-98.
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