Macrophage Colony-Stimulating Factor in Human Fetal Astrocytes and Microglia

Differential Regulation by Cytokines and Lipopolysaccharide, and Modulation of Class II MHC on Microglia

Sunhee C. Lee, Wei Liu, Philip Roth, Dennis W Dickson, Joan W. Berman, Celia F. Brosnan

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

CSF-1 is a growth factor that selectively promotes the proliferation, survival, and differentiation of cells of the mononuclear phagocyte series. As part of a study on the role of cytokine and hematopoietic growth factors in central nervous system (CNS) development and inflammation, we examined the expression of CSF-1 in dissociated glial cells cultured from human fetal CNS tissue. CSF-1 mRNA and protein were constitutively expressed by astrocytes. The steady state level of CSF-1 mRNA was markedly up-regulated by both IL-1β and TNF-α in a time- and dose-dependent manner, whereas only a minimal increase was detected after stimulation with IPS. In unstimulated astrocyte cultures, CSF-1 protein levels gradually increased to 3.5-fold base-line values by 96 h and were significantly increased by all three stimulants in the order of IL-1 ≥ TNF > IPS. Low levels of CSF-1 mRNA and protein were also detected in unstimulated microglia cultures. In contrast to astrocyte cultures, CSF-1 mRNA and protein increased significantly after stimulation with LPS, but changed only minimally after exposure to TNF-α or IL-1β. The effect of CSF-1 on cell proliferation, morphology, and class II MHC Ag expression was determined in highly enriched cultures of microglia and astrocytes. Microglia treated with CSF-1 showed a modest level of proliferation and differentiation into rod-shaped cells, whereas neither cell number nor shape was changed in astrocyte cultures. Interestingly, marked inhibition of both basal and IFNγ-induced class II MHC Ag expression was observed in microglial cells cultured in the presence of CSF-1, whereas no effect was detected in astrocytes. These results suggest the possibility that in situ production of CSF-1 in the CNS may regulate normal glial cell development and contribute to the immunologic status of the CNS through the down-regulation of class II MHC expression.

Original languageEnglish (US)
Pages (from-to)594-604
Number of pages11
JournalJournal of Immunology
Volume150
Issue number2
StatePublished - 1993
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Microglia
Astrocytes
Lipopolysaccharides
Cytokines
Central Nervous System
Interleukin-1
Messenger RNA
Neuroglia
Intercellular Signaling Peptides and Proteins
Proteins
Nerve Tissue
Phagocytes
Cell Differentiation
Cultured Cells
Cell Survival
Down-Regulation
Cell Count
Cell Proliferation
Inflammation

ASJC Scopus subject areas

  • Immunology

Cite this

Macrophage Colony-Stimulating Factor in Human Fetal Astrocytes and Microglia : Differential Regulation by Cytokines and Lipopolysaccharide, and Modulation of Class II MHC on Microglia. / Lee, Sunhee C.; Liu, Wei; Roth, Philip; Dickson, Dennis W; Berman, Joan W.; Brosnan, Celia F.

In: Journal of Immunology, Vol. 150, No. 2, 1993, p. 594-604.

Research output: Contribution to journalArticle

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