Loss of 18q and homozygosity for the DCC locus: Possible markers for clinically aggressive squamous cell carcinoma

Wolfgang Kelker, Daniel L. Van Dyke, Maria J. Worsham, Patricia L. Christopherson, C. David James, Margaret R. Conlon, Thomas E. Carey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Karyotyping and polymorphisms within the DCC (deleted in colon cancer) locus (18q21) were used to analyse loss of chromosome 18 in squamous cell carcinomas (SCC). Tumors from 26 patients (including 7 for whom matched tumor and normal DNA samples were available) were examined for heterozygous (LOH) at DCC. For 19 SCC tumor cultures normal tissue was not available. These were scored only as homozygous or heterozygous. The majority were homozygous. Only 3/19 (15%) were heterozygous. In contrast, in a panel of normal blood samples the majority, 11/16 (69%), were heterozygous. Allelic zygosity was concordant with the chromosome 18 content in the 16 tumors that were also karyotyped. Tumours from 40 patients, 37 that were karyotyped and three that were informative at the DCC locus, were assessed for loss of chromosome 18 occurred in tumours from 25. Twenty seven of the 40 patients have died and 13 are alive. There was strong association between loss of 18 and overall survival. Of those who are alive only 5/13 (38%) had loss of 18, whereas among those who have died 20/27 (74%) had loss of 18. By χ2 analysis the association of loss of 18 and death from cancer was significant (p > 0.01). The high frequency of chromosome 18 loss in SCC suggests that this region contains one or more tumor suppressor genes important in the clinical behaviour of SCC. DCC is one candidate, but other regions of loss not including the DCC locus indicate the chromosome 18 probably contains more than one tumor supressor locus. Prospective studies of chromosome 18 loss as a single prognostic indicator are strongly indicated in this tumor type since loss in early stage tumors might indicate a need for more aggressive therapy than would be given on the basis of staying alone.

Original languageEnglish (US)
Pages (from-to)2365-2372
Number of pages8
JournalAnticancer Research
Volume16
Issue number4 C
StatePublished - Jul 1996
Externally publishedYes

Fingerprint

Colonic Neoplasms
Squamous Cell Carcinoma
Chromosomes, Human, Pair 18
Neoplasms
Karyotyping
Tumor Suppressor Genes
Prospective Studies
Survival
DNA

Keywords

  • Chromosomes
  • DCC locus
  • Homozygosity
  • Loss
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kelker, W., Van Dyke, D. L., Worsham, M. J., Christopherson, P. L., James, C. D., Conlon, M. R., & Carey, T. E. (1996). Loss of 18q and homozygosity for the DCC locus: Possible markers for clinically aggressive squamous cell carcinoma. Anticancer Research, 16(4 C), 2365-2372.

Loss of 18q and homozygosity for the DCC locus : Possible markers for clinically aggressive squamous cell carcinoma. / Kelker, Wolfgang; Van Dyke, Daniel L.; Worsham, Maria J.; Christopherson, Patricia L.; James, C. David; Conlon, Margaret R.; Carey, Thomas E.

In: Anticancer Research, Vol. 16, No. 4 C, 07.1996, p. 2365-2372.

Research output: Contribution to journalArticle

Kelker, W, Van Dyke, DL, Worsham, MJ, Christopherson, PL, James, CD, Conlon, MR & Carey, TE 1996, 'Loss of 18q and homozygosity for the DCC locus: Possible markers for clinically aggressive squamous cell carcinoma', Anticancer Research, vol. 16, no. 4 C, pp. 2365-2372.
Kelker W, Van Dyke DL, Worsham MJ, Christopherson PL, James CD, Conlon MR et al. Loss of 18q and homozygosity for the DCC locus: Possible markers for clinically aggressive squamous cell carcinoma. Anticancer Research. 1996 Jul;16(4 C):2365-2372.
Kelker, Wolfgang ; Van Dyke, Daniel L. ; Worsham, Maria J. ; Christopherson, Patricia L. ; James, C. David ; Conlon, Margaret R. ; Carey, Thomas E. / Loss of 18q and homozygosity for the DCC locus : Possible markers for clinically aggressive squamous cell carcinoma. In: Anticancer Research. 1996 ; Vol. 16, No. 4 C. pp. 2365-2372.
@article{75cbec01af354e9d94ad80c8ae3818d4,
title = "Loss of 18q and homozygosity for the DCC locus: Possible markers for clinically aggressive squamous cell carcinoma",
abstract = "Karyotyping and polymorphisms within the DCC (deleted in colon cancer) locus (18q21) were used to analyse loss of chromosome 18 in squamous cell carcinomas (SCC). Tumors from 26 patients (including 7 for whom matched tumor and normal DNA samples were available) were examined for heterozygous (LOH) at DCC. For 19 SCC tumor cultures normal tissue was not available. These were scored only as homozygous or heterozygous. The majority were homozygous. Only 3/19 (15{\%}) were heterozygous. In contrast, in a panel of normal blood samples the majority, 11/16 (69{\%}), were heterozygous. Allelic zygosity was concordant with the chromosome 18 content in the 16 tumors that were also karyotyped. Tumours from 40 patients, 37 that were karyotyped and three that were informative at the DCC locus, were assessed for loss of chromosome 18 occurred in tumours from 25. Twenty seven of the 40 patients have died and 13 are alive. There was strong association between loss of 18 and overall survival. Of those who are alive only 5/13 (38{\%}) had loss of 18, whereas among those who have died 20/27 (74{\%}) had loss of 18. By χ2 analysis the association of loss of 18 and death from cancer was significant (p > 0.01). The high frequency of chromosome 18 loss in SCC suggests that this region contains one or more tumor suppressor genes important in the clinical behaviour of SCC. DCC is one candidate, but other regions of loss not including the DCC locus indicate the chromosome 18 probably contains more than one tumor supressor locus. Prospective studies of chromosome 18 loss as a single prognostic indicator are strongly indicated in this tumor type since loss in early stage tumors might indicate a need for more aggressive therapy than would be given on the basis of staying alone.",
keywords = "Chromosomes, DCC locus, Homozygosity, Loss, Squamous cell carcinoma",
author = "Wolfgang Kelker and {Van Dyke}, {Daniel L.} and Worsham, {Maria J.} and Christopherson, {Patricia L.} and James, {C. David} and Conlon, {Margaret R.} and Carey, {Thomas E.}",
year = "1996",
month = "7",
language = "English (US)",
volume = "16",
pages = "2365--2372",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "4 C",

}

TY - JOUR

T1 - Loss of 18q and homozygosity for the DCC locus

T2 - Possible markers for clinically aggressive squamous cell carcinoma

AU - Kelker, Wolfgang

AU - Van Dyke, Daniel L.

AU - Worsham, Maria J.

AU - Christopherson, Patricia L.

AU - James, C. David

AU - Conlon, Margaret R.

AU - Carey, Thomas E.

PY - 1996/7

Y1 - 1996/7

N2 - Karyotyping and polymorphisms within the DCC (deleted in colon cancer) locus (18q21) were used to analyse loss of chromosome 18 in squamous cell carcinomas (SCC). Tumors from 26 patients (including 7 for whom matched tumor and normal DNA samples were available) were examined for heterozygous (LOH) at DCC. For 19 SCC tumor cultures normal tissue was not available. These were scored only as homozygous or heterozygous. The majority were homozygous. Only 3/19 (15%) were heterozygous. In contrast, in a panel of normal blood samples the majority, 11/16 (69%), were heterozygous. Allelic zygosity was concordant with the chromosome 18 content in the 16 tumors that were also karyotyped. Tumours from 40 patients, 37 that were karyotyped and three that were informative at the DCC locus, were assessed for loss of chromosome 18 occurred in tumours from 25. Twenty seven of the 40 patients have died and 13 are alive. There was strong association between loss of 18 and overall survival. Of those who are alive only 5/13 (38%) had loss of 18, whereas among those who have died 20/27 (74%) had loss of 18. By χ2 analysis the association of loss of 18 and death from cancer was significant (p > 0.01). The high frequency of chromosome 18 loss in SCC suggests that this region contains one or more tumor suppressor genes important in the clinical behaviour of SCC. DCC is one candidate, but other regions of loss not including the DCC locus indicate the chromosome 18 probably contains more than one tumor supressor locus. Prospective studies of chromosome 18 loss as a single prognostic indicator are strongly indicated in this tumor type since loss in early stage tumors might indicate a need for more aggressive therapy than would be given on the basis of staying alone.

AB - Karyotyping and polymorphisms within the DCC (deleted in colon cancer) locus (18q21) were used to analyse loss of chromosome 18 in squamous cell carcinomas (SCC). Tumors from 26 patients (including 7 for whom matched tumor and normal DNA samples were available) were examined for heterozygous (LOH) at DCC. For 19 SCC tumor cultures normal tissue was not available. These were scored only as homozygous or heterozygous. The majority were homozygous. Only 3/19 (15%) were heterozygous. In contrast, in a panel of normal blood samples the majority, 11/16 (69%), were heterozygous. Allelic zygosity was concordant with the chromosome 18 content in the 16 tumors that were also karyotyped. Tumours from 40 patients, 37 that were karyotyped and three that were informative at the DCC locus, were assessed for loss of chromosome 18 occurred in tumours from 25. Twenty seven of the 40 patients have died and 13 are alive. There was strong association between loss of 18 and overall survival. Of those who are alive only 5/13 (38%) had loss of 18, whereas among those who have died 20/27 (74%) had loss of 18. By χ2 analysis the association of loss of 18 and death from cancer was significant (p > 0.01). The high frequency of chromosome 18 loss in SCC suggests that this region contains one or more tumor suppressor genes important in the clinical behaviour of SCC. DCC is one candidate, but other regions of loss not including the DCC locus indicate the chromosome 18 probably contains more than one tumor supressor locus. Prospective studies of chromosome 18 loss as a single prognostic indicator are strongly indicated in this tumor type since loss in early stage tumors might indicate a need for more aggressive therapy than would be given on the basis of staying alone.

KW - Chromosomes

KW - DCC locus

KW - Homozygosity

KW - Loss

KW - Squamous cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=0029789485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029789485&partnerID=8YFLogxK

M3 - Article

C2 - 8816836

AN - SCOPUS:0029789485

VL - 16

SP - 2365

EP - 2372

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 4 C

ER -