Long-term perfusion of the cerebroventricular system of dogs without leakage to the peripheral circulation

S. Nitecki, R. Karmeli, G. J. Harty, C. Kamei, T. L. Yaksh, J. H. Szurszewski

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Methods developed previously for studying the effect of cerebroventricular injection or ventriculocisternal perfusion of test substances are unsatisfactory because the test substance is not confined to the central compartment. Most likely the test substance enters the peripheral circulation via the arachnoid villi. The purpose of this paper is to describe a method for perfusing the cerebroventricular system of conscious dogs without passage of test substances to the peripheral circulation. With the method described, the mean (±SE) cerebroventricular pressure in conscious dogs was 7.4 ± 0.8 cmH2O (n = 16), and the mean (±SE) production of cerebrospinal fluid (CSF) was 25 ± 0.3 μl/min (n = 16). Endogenously occurring migrating myoelectric complexes (MMCs) of the small intestine were recorded in dogs before catheters were implanted in the left and right lateral ventricles and the fourth ventricle and after catheter implantation during cerebroventricular perfusion with artificial CSF alone or with CSF containing sulfated (S-CCK- OP) or nonsulfated cholecystokinin octapeptide (NS-CCK-OP). Only cerebroventricular perfusion with S-CCK-OP (1.2 pmol · kg-1 · min-1; n = 20) replaced spontaneously occurring MMCs with a fed-like pattern of myoelectric activity. The results suggest that replacement of the fasting pattern of myoelectric activity with a fed-like pattern in the fasted dog was mediated by CCK-A receptors located in one or more brain nuclei surrounding the third ventricle.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume267
Issue number5 36-5
StatePublished - 1994

Fingerprint

Perfusion
Migrating Myoelectric Complexes
Dogs
Cerebrospinal Fluid
Catheters
Cholecystokinin A Receptor
Arachnoid
Fourth Ventricle
Sincalide
Third Ventricle
Lateral Ventricles
Small Intestine
Heart Ventricles
Fasting
Pressure
Injections
Brain
8-sulfocholecystokinin octapeptide

Keywords

  • cerebroventricular perfusion
  • cerebroventricular pressure
  • cholecystokinin octapeptide
  • gut myoelectrical activity
  • migrating myoelectric complex

ASJC Scopus subject areas

  • Physiology

Cite this

Long-term perfusion of the cerebroventricular system of dogs without leakage to the peripheral circulation. / Nitecki, S.; Karmeli, R.; Harty, G. J.; Kamei, C.; Yaksh, T. L.; Szurszewski, J. H.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 267, No. 5 36-5, 1994.

Research output: Contribution to journalArticle

Nitecki, S. ; Karmeli, R. ; Harty, G. J. ; Kamei, C. ; Yaksh, T. L. ; Szurszewski, J. H. / Long-term perfusion of the cerebroventricular system of dogs without leakage to the peripheral circulation. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 1994 ; Vol. 267, No. 5 36-5.
@article{a9a8f92fa8b949c08917e1deed37e027,
title = "Long-term perfusion of the cerebroventricular system of dogs without leakage to the peripheral circulation",
abstract = "Methods developed previously for studying the effect of cerebroventricular injection or ventriculocisternal perfusion of test substances are unsatisfactory because the test substance is not confined to the central compartment. Most likely the test substance enters the peripheral circulation via the arachnoid villi. The purpose of this paper is to describe a method for perfusing the cerebroventricular system of conscious dogs without passage of test substances to the peripheral circulation. With the method described, the mean (±SE) cerebroventricular pressure in conscious dogs was 7.4 ± 0.8 cmH2O (n = 16), and the mean (±SE) production of cerebrospinal fluid (CSF) was 25 ± 0.3 μl/min (n = 16). Endogenously occurring migrating myoelectric complexes (MMCs) of the small intestine were recorded in dogs before catheters were implanted in the left and right lateral ventricles and the fourth ventricle and after catheter implantation during cerebroventricular perfusion with artificial CSF alone or with CSF containing sulfated (S-CCK- OP) or nonsulfated cholecystokinin octapeptide (NS-CCK-OP). Only cerebroventricular perfusion with S-CCK-OP (1.2 pmol · kg-1 · min-1; n = 20) replaced spontaneously occurring MMCs with a fed-like pattern of myoelectric activity. The results suggest that replacement of the fasting pattern of myoelectric activity with a fed-like pattern in the fasted dog was mediated by CCK-A receptors located in one or more brain nuclei surrounding the third ventricle.",
keywords = "cerebroventricular perfusion, cerebroventricular pressure, cholecystokinin octapeptide, gut myoelectrical activity, migrating myoelectric complex",
author = "S. Nitecki and R. Karmeli and Harty, {G. J.} and C. Kamei and Yaksh, {T. L.} and Szurszewski, {J. H.}",
year = "1994",
language = "English (US)",
volume = "267",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "5 36-5",

}

TY - JOUR

T1 - Long-term perfusion of the cerebroventricular system of dogs without leakage to the peripheral circulation

AU - Nitecki, S.

AU - Karmeli, R.

AU - Harty, G. J.

AU - Kamei, C.

AU - Yaksh, T. L.

AU - Szurszewski, J. H.

PY - 1994

Y1 - 1994

N2 - Methods developed previously for studying the effect of cerebroventricular injection or ventriculocisternal perfusion of test substances are unsatisfactory because the test substance is not confined to the central compartment. Most likely the test substance enters the peripheral circulation via the arachnoid villi. The purpose of this paper is to describe a method for perfusing the cerebroventricular system of conscious dogs without passage of test substances to the peripheral circulation. With the method described, the mean (±SE) cerebroventricular pressure in conscious dogs was 7.4 ± 0.8 cmH2O (n = 16), and the mean (±SE) production of cerebrospinal fluid (CSF) was 25 ± 0.3 μl/min (n = 16). Endogenously occurring migrating myoelectric complexes (MMCs) of the small intestine were recorded in dogs before catheters were implanted in the left and right lateral ventricles and the fourth ventricle and after catheter implantation during cerebroventricular perfusion with artificial CSF alone or with CSF containing sulfated (S-CCK- OP) or nonsulfated cholecystokinin octapeptide (NS-CCK-OP). Only cerebroventricular perfusion with S-CCK-OP (1.2 pmol · kg-1 · min-1; n = 20) replaced spontaneously occurring MMCs with a fed-like pattern of myoelectric activity. The results suggest that replacement of the fasting pattern of myoelectric activity with a fed-like pattern in the fasted dog was mediated by CCK-A receptors located in one or more brain nuclei surrounding the third ventricle.

AB - Methods developed previously for studying the effect of cerebroventricular injection or ventriculocisternal perfusion of test substances are unsatisfactory because the test substance is not confined to the central compartment. Most likely the test substance enters the peripheral circulation via the arachnoid villi. The purpose of this paper is to describe a method for perfusing the cerebroventricular system of conscious dogs without passage of test substances to the peripheral circulation. With the method described, the mean (±SE) cerebroventricular pressure in conscious dogs was 7.4 ± 0.8 cmH2O (n = 16), and the mean (±SE) production of cerebrospinal fluid (CSF) was 25 ± 0.3 μl/min (n = 16). Endogenously occurring migrating myoelectric complexes (MMCs) of the small intestine were recorded in dogs before catheters were implanted in the left and right lateral ventricles and the fourth ventricle and after catheter implantation during cerebroventricular perfusion with artificial CSF alone or with CSF containing sulfated (S-CCK- OP) or nonsulfated cholecystokinin octapeptide (NS-CCK-OP). Only cerebroventricular perfusion with S-CCK-OP (1.2 pmol · kg-1 · min-1; n = 20) replaced spontaneously occurring MMCs with a fed-like pattern of myoelectric activity. The results suggest that replacement of the fasting pattern of myoelectric activity with a fed-like pattern in the fasted dog was mediated by CCK-A receptors located in one or more brain nuclei surrounding the third ventricle.

KW - cerebroventricular perfusion

KW - cerebroventricular pressure

KW - cholecystokinin octapeptide

KW - gut myoelectrical activity

KW - migrating myoelectric complex

UR - http://www.scopus.com/inward/record.url?scp=0028144082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028144082&partnerID=8YFLogxK

M3 - Article

C2 - 7977859

AN - SCOPUS:0028144082

VL - 267

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 5 36-5

ER -