Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter

M. W. Berchtold; M. Koller; R. Egli; J. A. Rhyner; H. Hameister; E. E. Strehler

doi:10.1007/BF00217447

Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter

M. W. Berchtold, M. Koller, R. Egli, J. A. Rhyner, H. Hameister, E. E. Strehler

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The functional intronless gene coding for a calmodulin-like protein (CLP) has been localized to human chromosome 10p13-ter. Chromosomal assignment was performed by Southern blot analysis of DNA from human-rodent somatic cell hybrids and amplification of a CLP gene-specific 1090-bp DNA fragment by the polymerase chain reaction (PCR) on DNA from human-hamster cell hybrids. Chromosomal sublocalization was carried out by in situ hybridization of human chromosome metaphase spreads. The CLP gene is the first member of the human calmodulin/calmodulin-like gene family to be chromosomally sublocalized. Its presence near the telomeric end of the short arm of chromosome 10 may be of significance with respect to its highly (epithelial) cell-type restricted expression in vivo and strong downregulation upon malignant transformation. The generation of a human CLP gene-specific sequence tag site specified by the two primers used for PCR should prove useful for future linkage studies.

Original language	English (US)
Pages (from-to)	496-500
Number of pages	5
Journal	Human genetics
Volume	90
Issue number	5
DOIs	https://doi.org/10.1007/BF00217447
State	Published - Jan 1993

ASJC Scopus subject areas

Genetics
Genetics(clinical)

Access to Document

10.1007/BF00217447

Cite this

@article{d607cfd30fb64ce99d010b1f323adbf3,

title = "Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter",

abstract = "The functional intronless gene coding for a calmodulin-like protein (CLP) has been localized to human chromosome 10p13-ter. Chromosomal assignment was performed by Southern blot analysis of DNA from human-rodent somatic cell hybrids and amplification of a CLP gene-specific 1090-bp DNA fragment by the polymerase chain reaction (PCR) on DNA from human-hamster cell hybrids. Chromosomal sublocalization was carried out by in situ hybridization of human chromosome metaphase spreads. The CLP gene is the first member of the human calmodulin/calmodulin-like gene family to be chromosomally sublocalized. Its presence near the telomeric end of the short arm of chromosome 10 may be of significance with respect to its highly (epithelial) cell-type restricted expression in vivo and strong downregulation upon malignant transformation. The generation of a human CLP gene-specific sequence tag site specified by the two primers used for PCR should prove useful for future linkage studies.",

author = "Berchtold, {M. W.} and M. Koller and R. Egli and Rhyner, {J. A.} and H. Hameister and Strehler, {E. E.}",

year = "1993",

month = jan,

doi = "10.1007/BF00217447",

language = "English (US)",

volume = "90",

pages = "496--500",

journal = "Human genetics",

issn = "0340-6717",

publisher = "Springer Verlag",

number = "5",

}

TY - JOUR

T1 - Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter

AU - Berchtold, M. W.

AU - Koller, M.

AU - Egli, R.

AU - Rhyner, J. A.

AU - Hameister, H.

AU - Strehler, E. E.

PY - 1993/1

Y1 - 1993/1

N2 - The functional intronless gene coding for a calmodulin-like protein (CLP) has been localized to human chromosome 10p13-ter. Chromosomal assignment was performed by Southern blot analysis of DNA from human-rodent somatic cell hybrids and amplification of a CLP gene-specific 1090-bp DNA fragment by the polymerase chain reaction (PCR) on DNA from human-hamster cell hybrids. Chromosomal sublocalization was carried out by in situ hybridization of human chromosome metaphase spreads. The CLP gene is the first member of the human calmodulin/calmodulin-like gene family to be chromosomally sublocalized. Its presence near the telomeric end of the short arm of chromosome 10 may be of significance with respect to its highly (epithelial) cell-type restricted expression in vivo and strong downregulation upon malignant transformation. The generation of a human CLP gene-specific sequence tag site specified by the two primers used for PCR should prove useful for future linkage studies.

AB - The functional intronless gene coding for a calmodulin-like protein (CLP) has been localized to human chromosome 10p13-ter. Chromosomal assignment was performed by Southern blot analysis of DNA from human-rodent somatic cell hybrids and amplification of a CLP gene-specific 1090-bp DNA fragment by the polymerase chain reaction (PCR) on DNA from human-hamster cell hybrids. Chromosomal sublocalization was carried out by in situ hybridization of human chromosome metaphase spreads. The CLP gene is the first member of the human calmodulin/calmodulin-like gene family to be chromosomally sublocalized. Its presence near the telomeric end of the short arm of chromosome 10 may be of significance with respect to its highly (epithelial) cell-type restricted expression in vivo and strong downregulation upon malignant transformation. The generation of a human CLP gene-specific sequence tag site specified by the two primers used for PCR should prove useful for future linkage studies.

UR - http://www.scopus.com/inward/record.url?scp=0027418953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027418953&partnerID=8YFLogxK

U2 - 10.1007/BF00217447

DO - 10.1007/BF00217447

M3 - Article

C2 - 8428750

AN - SCOPUS:0027418953

SN - 0340-6717

VL - 90

SP - 496

EP - 500

JO - Human genetics

JF - Human genetics

IS - 5

ER -

Localization of the intronless gene coding for calmodulin-like protein CLP to human chromosome 10p13-ter

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this