Local structure of a peptide contact site on a(Formula presented)

Bing Yuan Wei, Nadine Gervois, Georges Mer, Luciano Adorini, Christophe Benoist, Diane Mathis

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have sought to determine how much amino acid diversity is tolerable at position 69 of the Ak chain, a position previously implicated as a peptide contact site. Slot-machine mutagenesis was used to create a set of 11 mutant Ak cDNAs, each specifying a different amino acid at position 69. These cDNAs were individually expressed In L cells together with a wild-type Ak cDNA to produce a panel of mutant antigen-presenting cell lines. The ability of each member of this panel to present a hen egg lysozyme and a bovine ribonucléase peptide to various T hybrldomas was assessed. We found that a surprising degree of amino acid diversity is tolerable at Ak position 69: Even charged (Glu, Arg) or bulky (Trp, Tyr) residues can be accommodated without abrogating cell-surface expression of Ak, peptide binding to it, or T cell recognition of it. We discuss the implications of these findings for models of T cell recognition of the class II molecule/antlgen duplex.

Original languageEnglish (US)
Pages (from-to)833-837
Number of pages5
JournalInternational Immunology
Volume3
Issue number8
DOIs
StatePublished - Aug 1991

Keywords

  • Antigen presentation
  • MHC class II
  • Mutagenesis
  • Structure/function

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Wei, B. Y., Gervois, N., Mer, G., Adorini, L., Benoist, C., & Mathis, D. (1991). Local structure of a peptide contact site on a(Formula presented). International Immunology, 3(8), 833-837. https://doi.org/10.1093/intimm/3.8.833