Lipid-protein interactions in lipovitellin

James R. Thompson, Leonard J. Banaszak

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The refined molecular structure of lipovitellin is described using synchrotron cryocrystallographic data to 1.9 Å resolution. Lipovitellin is the predominant lipoprotein found in the yolk of egg-laying animals and is involved in lipid and metal storage. It is thought to be related in amino acid sequence to segments of apolipoprotein B and the microsomal transfer protein responsible for the assembly of low-density lipoproteins. Lipovitellin contains a heterogeneous mixture of about 16% (w/w) noncovalently bound lipid, mostly phospholipid. Previous X-ray structural studies at ambient temperature described several different protein domains including a large cavity in each subunit of the dimeric protein. The cavity was free of any visible electron density for lipid molecules at room temperature, suggesting that only dynamic interactions exist with the protein. An important result from this crystallographic study at 100 K is the appearance of some bound ordered lipid along the walls of the binding cavity. The precise identification of the lipid type is difficult because of discontinuities in the electron density. Nonetheless, the conformations of 7 phospholipids and 43 segments of hydrocarbon chains greater than 5 atoms in length have been discovered. The conformations of the bound lipid and the interactions between protein and lipid provide insights into the factors governing lipoprotein formation.

Original languageEnglish (US)
Pages (from-to)9398-9409
Number of pages12
JournalBiochemistry
Volume41
Issue number30
DOIs
StatePublished - Jul 30 2002
Externally publishedYes

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Lipids
Proteins
Lipoproteins
Carrier concentration
Conformations
Phospholipids
Electrons
Synchrotrons
Egg Yolk
Temperature
Protein Subunits
Apolipoproteins B
Hydrocarbons
lipovitellin
Molecular Structure
LDL Lipoproteins
Molecular structure
Amino Acid Sequence
Animals
Metals

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thompson, J. R., & Banaszak, L. J. (2002). Lipid-protein interactions in lipovitellin. Biochemistry, 41(30), 9398-9409. https://doi.org/10.1021/bi025674w

Lipid-protein interactions in lipovitellin. / Thompson, James R.; Banaszak, Leonard J.

In: Biochemistry, Vol. 41, No. 30, 30.07.2002, p. 9398-9409.

Research output: Contribution to journalArticle

Thompson, JR & Banaszak, LJ 2002, 'Lipid-protein interactions in lipovitellin', Biochemistry, vol. 41, no. 30, pp. 9398-9409. https://doi.org/10.1021/bi025674w
Thompson JR, Banaszak LJ. Lipid-protein interactions in lipovitellin. Biochemistry. 2002 Jul 30;41(30):9398-9409. https://doi.org/10.1021/bi025674w
Thompson, James R. ; Banaszak, Leonard J. / Lipid-protein interactions in lipovitellin. In: Biochemistry. 2002 ; Vol. 41, No. 30. pp. 9398-9409.
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