Linking DNA Methyltransferases to Epigenetic Marks and Nucleosome Structure Genome-wide in Human Tumor Cells

Bilian Jin, Jason Ernst, Rochelle L. Tiedemann, Hongyan Xu, Suhas Sureshchandra, Manolis Kellis, Stephen Dalton, Chen Liu, Jeong Hyeon Choi, Keith D Robertson

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

DNA methylation, mediated by the combined action of three DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), is essential for mammalian development and is a major contributor to cellular transformation. To elucidate how DNA methylation is targeted, we mapped the genome-wide localization of all DNMTs and methylation, and examined the relationships among these markers, histone modifications, and nucleosome structure in a pluripotent human tumor cell line in its undifferentiated and differentiated states. Our findings reveal a strong link between DNMTs and transcribed loci, and that DNA methylation is not a simple sum of DNMT localization patterns. A comparison of the epigenomes of normal and cancerous stem cells, and pluripotent and differentiated states shows that the presence of at least two DNMTs is strongly associated with loci targeted for DNA hypermethylation. Taken together, these results shed important light on the determinants of DNA methylation and how it may become disrupted in cancer cells.

Original languageEnglish (US)
Pages (from-to)1411-1424
Number of pages14
JournalCell Reports
Volume2
Issue number5
DOIs
StatePublished - Nov 29 2012
Externally publishedYes

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Nucleosomes
Methyltransferases
DNA Methylation
Epigenomics
Tumors
Genes
Cells
Genome
DNA
Neoplasms
Histone Code
Pluripotent Stem Cells
Methylation
Stem cells
Tumor Cell Line
Histones

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Linking DNA Methyltransferases to Epigenetic Marks and Nucleosome Structure Genome-wide in Human Tumor Cells. / Jin, Bilian; Ernst, Jason; Tiedemann, Rochelle L.; Xu, Hongyan; Sureshchandra, Suhas; Kellis, Manolis; Dalton, Stephen; Liu, Chen; Choi, Jeong Hyeon; Robertson, Keith D.

In: Cell Reports, Vol. 2, No. 5, 29.11.2012, p. 1411-1424.

Research output: Contribution to journalArticle

Jin, B, Ernst, J, Tiedemann, RL, Xu, H, Sureshchandra, S, Kellis, M, Dalton, S, Liu, C, Choi, JH & Robertson, KD 2012, 'Linking DNA Methyltransferases to Epigenetic Marks and Nucleosome Structure Genome-wide in Human Tumor Cells', Cell Reports, vol. 2, no. 5, pp. 1411-1424. https://doi.org/10.1016/j.celrep.2012.10.017
Jin, Bilian ; Ernst, Jason ; Tiedemann, Rochelle L. ; Xu, Hongyan ; Sureshchandra, Suhas ; Kellis, Manolis ; Dalton, Stephen ; Liu, Chen ; Choi, Jeong Hyeon ; Robertson, Keith D. / Linking DNA Methyltransferases to Epigenetic Marks and Nucleosome Structure Genome-wide in Human Tumor Cells. In: Cell Reports. 2012 ; Vol. 2, No. 5. pp. 1411-1424.
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