Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation

Timothy Mark Olson, Alexey E. Alekseev, Xiaoke K. Liu, Sungjo Park, Leonid V. Zingman, Martin Bienengraeber, Srinivasan Sattiraju, Jeffrey D. Ballew, Arshad Jahangir, Andre Terzic

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

Atrial fibrillation is a rhythm disorder characterized by chaotic electrical activity of cardiac atria. Predisposing to stroke and heart failure, this common condition is increasingly recognized as a heritable disorder. To identify genetic defects conferring disease susceptibility, patients with idiopathic atrial fibrillation, lacking traditional risk factors, were evaluated. Genomic DNA scanning revealed a nonsense mutation in KCNA5 that encodes Kv1.5, a voltage-gated potassium channel expressed in human atria. The heterozygous E375X mutation, present in a familial case of atrial fibrillation and absent in 540 unrelated control individuals, introduced a premature stop codon disrupting the Kv1.5 channel protein. The truncation eliminated the S4-S6 voltage sensor, pore region and C-terminus, preserving the N-terminus and S1-S3 transmembrane domains that secure tetrameric subunit assembly. Heterologously expressed recombinant E375X mutant failed to generate the ultrarapid delayed rectifier current IKur vital for atrial repolarization and exerted a dominant-negative effect on wild-type current. Loss of channel function translated into action potential prolongation and early after-depolarization in human atrial myocytes, increasing vulnerability to stress-provoked triggered activity. The pathogenic link between compromised Kv1.5 function and susceptibility to atrial fibrillation was verified, at the organism level, in a murine model. Rescue of the genetic defect was achieved by aminoglycoside-induced translational read-through of the E375X premature stop codon, restoring channel function. This first report of Kv1.5 loss-of-function channelopathy establishes KCNA5 mutation as a novel risk factor for repolarization deficiency and atrial fibrillation.

Original languageEnglish (US)
Pages (from-to)2185-2191
Number of pages7
JournalHuman Molecular Genetics
Volume15
Issue number14
DOIs
StatePublished - Jul 15 2006

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Channelopathies
Atrial Fibrillation
Nonsense Codon
Mutation
Voltage-Gated Potassium Channels
S 6
Disease Susceptibility
Aminoglycosides
Muscle Cells
Action Potentials
Heart Failure
Stroke
DNA
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation. / Olson, Timothy Mark; Alekseev, Alexey E.; Liu, Xiaoke K.; Park, Sungjo; Zingman, Leonid V.; Bienengraeber, Martin; Sattiraju, Srinivasan; Ballew, Jeffrey D.; Jahangir, Arshad; Terzic, Andre.

In: Human Molecular Genetics, Vol. 15, No. 14, 15.07.2006, p. 2185-2191.

Research output: Contribution to journalArticle

Olson, TM, Alekseev, AE, Liu, XK, Park, S, Zingman, LV, Bienengraeber, M, Sattiraju, S, Ballew, JD, Jahangir, A & Terzic, A 2006, 'Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation', Human Molecular Genetics, vol. 15, no. 14, pp. 2185-2191. https://doi.org/10.1093/hmg/ddl143
Olson, Timothy Mark ; Alekseev, Alexey E. ; Liu, Xiaoke K. ; Park, Sungjo ; Zingman, Leonid V. ; Bienengraeber, Martin ; Sattiraju, Srinivasan ; Ballew, Jeffrey D. ; Jahangir, Arshad ; Terzic, Andre. / Kv1.5 channelopathy due to KCNA5 loss-of-function mutation causes human atrial fibrillation. In: Human Molecular Genetics. 2006 ; Vol. 15, No. 14. pp. 2185-2191.
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AU - Bienengraeber, Martin

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