Kinetics of the apoptotic response induced by anti-IgM engagement of the B cell receptor is dependent on the density of cell surface immunoglobulin M expression

Miguel A. Trujillo, Norman L. Eberhardt

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The effect of B cell receptor (BCR) density on anti-BCR-induced apoptosis was assessed in Ramos cell lines, expressing low, medium, or high levels of surface IgM (sIgMLO, sIgMMED, sIgMHI). All cells required a 6-μg/ml threshold of anti-IgM to elicit apoptosis. Anti-IgM treatment of sIgMLO cells induced growth inhibition and limited dose-independent apoptosis. Anti-IgM treatment of sIgMMED cells induced dose-independent death with a 32-h lag. Ligation of the BCR in the sIgMHI cells induced rapid apoptosis beginning by 6 h, which was dose-dependent. Secondary crosslinking reagents did not affect apoptosis, and this effect was independent of anti-IgM concentration, time, or sIgM density. These results suggest that the response to BCR engagement strongly depends on the cell surface receptor density.

Original languageEnglish (US)
Pages (from-to)525-531
Number of pages7
JournalDNA and Cell Biology
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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