Keratinocyte Growth Factor Causes Proliferation of Urothelium In Vivo

Eunhee S. Yi, Ahmed S. Shabaik, David L. Lacey, Adriana A. Bedoya, Songmei Yin, Regina M. Housley, Dimitry M. Danilenko, William Benson, Arthur M. Cohen, Glenn F. Pierce, Arlen Thomason, Thomas R. Ulich

Research output: Contribution to journalArticle

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Abstract

Purpose: To determine the effect of keratinocyte growth factor (KGF), a mesenchymally derived epithelial growth factor that can cause proliferation of pulmonary, gastrointestinal and mammary epithelia, on urothelium. Materials and Methods: Recombinant human KGF was systemically administered to rats and Rhesus monkeys, and the proliferative effects on the bladder were evaluated. Results: Keratinocyte growth factor causes proliferation of transitional epithelial cells. Proliferating cell nuclear antigen (PCNA) expression in rat bladder is dramatically increased along the basal layer of urothelium 1, 3, 7 and 14 days after daily injections of KGF. Incorporation of 5-bromodeoxyuridine (BrdU) at 7 and 14 days in the urothelium of KGF-treated rats parallels PCNA immunoreactivity and confirms that KGF increases DNA synthesis in urothelial cells. Urothelial cell proliferation is accompanied histologically by an increase in mitotic activity. Keratinocyte growth factor-induced PCNA expression is reversible upon cessation of KGF administration. Keratinocyte growth factor mRNA and receptor mRNA are detected by whole organ RNAse protection assays of the urinary bladder and the kidney of normal rats. Rhesus monkeys receiving KGF for 7 days demonstrate a dramatic incorporation of BrdU in the urothelium of the bladder and renal pelvis as well as in the collecting ducts of the kidney. Conclusion: Systemic administration of KGF causes rapid and striking proliferation of urothelium.

Original languageEnglish (US)
Pages (from-to)1566-1570
Number of pages5
JournalThe Journal of Urology
Volume154
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

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Fibroblast Growth Factor 7
Urothelium
Proliferating Cell Nuclear Antigen
Urinary Bladder
Bromodeoxyuridine
Macaca mulatta
Collecting Kidney Tubules
Messenger RNA
Kidney Pelvis
Intercellular Signaling Peptides and Proteins
Breast
Epithelium
Epithelial Cells
Cell Proliferation
Kidney

ASJC Scopus subject areas

  • Urology

Cite this

Yi, E. S., Shabaik, A. S., Lacey, D. L., Bedoya, A. A., Yin, S., Housley, R. M., ... Ulich, T. R. (1995). Keratinocyte Growth Factor Causes Proliferation of Urothelium In Vivo. The Journal of Urology, 154(4), 1566-1570. https://doi.org/10.1016/S0022-5347(01)66930-1

Keratinocyte Growth Factor Causes Proliferation of Urothelium In Vivo. / Yi, Eunhee S.; Shabaik, Ahmed S.; Lacey, David L.; Bedoya, Adriana A.; Yin, Songmei; Housley, Regina M.; Danilenko, Dimitry M.; Benson, William; Cohen, Arthur M.; Pierce, Glenn F.; Thomason, Arlen; Ulich, Thomas R.

In: The Journal of Urology, Vol. 154, No. 4, 1995, p. 1566-1570.

Research output: Contribution to journalArticle

Yi, ES, Shabaik, AS, Lacey, DL, Bedoya, AA, Yin, S, Housley, RM, Danilenko, DM, Benson, W, Cohen, AM, Pierce, GF, Thomason, A & Ulich, TR 1995, 'Keratinocyte Growth Factor Causes Proliferation of Urothelium In Vivo', The Journal of Urology, vol. 154, no. 4, pp. 1566-1570. https://doi.org/10.1016/S0022-5347(01)66930-1
Yi ES, Shabaik AS, Lacey DL, Bedoya AA, Yin S, Housley RM et al. Keratinocyte Growth Factor Causes Proliferation of Urothelium In Vivo. The Journal of Urology. 1995;154(4):1566-1570. https://doi.org/10.1016/S0022-5347(01)66930-1
Yi, Eunhee S. ; Shabaik, Ahmed S. ; Lacey, David L. ; Bedoya, Adriana A. ; Yin, Songmei ; Housley, Regina M. ; Danilenko, Dimitry M. ; Benson, William ; Cohen, Arthur M. ; Pierce, Glenn F. ; Thomason, Arlen ; Ulich, Thomas R. / Keratinocyte Growth Factor Causes Proliferation of Urothelium In Vivo. In: The Journal of Urology. 1995 ; Vol. 154, No. 4. pp. 1566-1570.
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abstract = "Purpose: To determine the effect of keratinocyte growth factor (KGF), a mesenchymally derived epithelial growth factor that can cause proliferation of pulmonary, gastrointestinal and mammary epithelia, on urothelium. Materials and Methods: Recombinant human KGF was systemically administered to rats and Rhesus monkeys, and the proliferative effects on the bladder were evaluated. Results: Keratinocyte growth factor causes proliferation of transitional epithelial cells. Proliferating cell nuclear antigen (PCNA) expression in rat bladder is dramatically increased along the basal layer of urothelium 1, 3, 7 and 14 days after daily injections of KGF. Incorporation of 5-bromodeoxyuridine (BrdU) at 7 and 14 days in the urothelium of KGF-treated rats parallels PCNA immunoreactivity and confirms that KGF increases DNA synthesis in urothelial cells. Urothelial cell proliferation is accompanied histologically by an increase in mitotic activity. Keratinocyte growth factor-induced PCNA expression is reversible upon cessation of KGF administration. Keratinocyte growth factor mRNA and receptor mRNA are detected by whole organ RNAse protection assays of the urinary bladder and the kidney of normal rats. Rhesus monkeys receiving KGF for 7 days demonstrate a dramatic incorporation of BrdU in the urothelium of the bladder and renal pelvis as well as in the collecting ducts of the kidney. Conclusion: Systemic administration of KGF causes rapid and striking proliferation of urothelium.",
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AU - Yi, Eunhee S.

AU - Shabaik, Ahmed S.

AU - Lacey, David L.

AU - Bedoya, Adriana A.

AU - Yin, Songmei

AU - Housley, Regina M.

AU - Danilenko, Dimitry M.

AU - Benson, William

AU - Cohen, Arthur M.

AU - Pierce, Glenn F.

AU - Thomason, Arlen

AU - Ulich, Thomas R.

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