Jejunal responses to absorptive and secretory stimuli in the neurally isolated jejunum in vivo

S. M. Herkes, C. D. Smith, M. G. Sarr

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background. Our aim was to assess changes in transport of water and electrolytes under basal, proabsorptive, and prosecretory conditions after an in situ neural isolation of the jejunoileum. Methods. Eight dogs underwent intraoperative perfusion of 50 cm of jejunum with a balanced electrolyte solution during sham operation and after neural isolation of the jejunoileum. Jejunal perfusion studies were later conducted in seven conscious dogs with a triple-lumen technique before and 2 and 8 weeks after neural isolation of the jejunoileum during intravenous infusion of 150 mmol/L sodium chloride (basal conditions), vasoactive intestinal polypeptide (VIP conditions), 500 pmol/kg per hour (prosecretory conditions), and the α2-adrenergic agonist α- methylnorepinephrine (MNE), 900 nmol/kg per hour (proabsorptive conditions). Results. Neural isolation decreased intraoperative net absorption of water (4.4 ± 0.9 vs 2.2 ± 0.5 μl/cm/min; p < 0.05) and electrolytes (sodium, chloride, bicarbonate, potassium). In conscious dogs during basal conditions, net absorptive flux was decreased (p < 0.05) at 2 but not at 8 weeks. VIP produced similar absolute decreases in net absorptive flux at all three time points. MNE increased net absorption before and at 8 weeks, but not at 2 weeks after autotransplantation. Conclusions. In situ neural isolation of the jejunoileum decreased net basal jejunal absorption of water and electrolytes immediately and for at least 2 weeks. Proabsorptive responses to MNE but not prosecretory responses to VIP were altered at 2 weeks. Jejunal adaptation allowed absorptive function to return to near normal by 8 weeks.

Original languageEnglish (US)
Pages (from-to)576-586
Number of pages11
JournalSurgery
Volume116
Issue number3
StatePublished - 1994

Fingerprint

Nordefrin
Jejunum
Electrolytes
Dogs
Sodium Chloride
Water
Perfusion
Adrenergic Agonists
Sodium Bicarbonate
Autologous Transplantation
Vasoactive Intestinal Peptide
Intravenous Infusions
Potassium

ASJC Scopus subject areas

  • Surgery

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Herkes, S. M., Smith, C. D., & Sarr, M. G. (1994). Jejunal responses to absorptive and secretory stimuli in the neurally isolated jejunum in vivo. Surgery, 116(3), 576-586.

Jejunal responses to absorptive and secretory stimuli in the neurally isolated jejunum in vivo. / Herkes, S. M.; Smith, C. D.; Sarr, M. G.

In: Surgery, Vol. 116, No. 3, 1994, p. 576-586.

Research output: Contribution to journalArticle

Herkes, SM, Smith, CD & Sarr, MG 1994, 'Jejunal responses to absorptive and secretory stimuli in the neurally isolated jejunum in vivo', Surgery, vol. 116, no. 3, pp. 576-586.
Herkes, S. M. ; Smith, C. D. ; Sarr, M. G. / Jejunal responses to absorptive and secretory stimuli in the neurally isolated jejunum in vivo. In: Surgery. 1994 ; Vol. 116, No. 3. pp. 576-586.
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N2 - Background. Our aim was to assess changes in transport of water and electrolytes under basal, proabsorptive, and prosecretory conditions after an in situ neural isolation of the jejunoileum. Methods. Eight dogs underwent intraoperative perfusion of 50 cm of jejunum with a balanced electrolyte solution during sham operation and after neural isolation of the jejunoileum. Jejunal perfusion studies were later conducted in seven conscious dogs with a triple-lumen technique before and 2 and 8 weeks after neural isolation of the jejunoileum during intravenous infusion of 150 mmol/L sodium chloride (basal conditions), vasoactive intestinal polypeptide (VIP conditions), 500 pmol/kg per hour (prosecretory conditions), and the α2-adrenergic agonist α- methylnorepinephrine (MNE), 900 nmol/kg per hour (proabsorptive conditions). Results. Neural isolation decreased intraoperative net absorption of water (4.4 ± 0.9 vs 2.2 ± 0.5 μl/cm/min; p < 0.05) and electrolytes (sodium, chloride, bicarbonate, potassium). In conscious dogs during basal conditions, net absorptive flux was decreased (p < 0.05) at 2 but not at 8 weeks. VIP produced similar absolute decreases in net absorptive flux at all three time points. MNE increased net absorption before and at 8 weeks, but not at 2 weeks after autotransplantation. Conclusions. In situ neural isolation of the jejunoileum decreased net basal jejunal absorption of water and electrolytes immediately and for at least 2 weeks. Proabsorptive responses to MNE but not prosecretory responses to VIP were altered at 2 weeks. Jejunal adaptation allowed absorptive function to return to near normal by 8 weeks.

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