Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Takayuki Miyazawa, Yasuhiro Ikeda, Kazuya Nakamura, Risako Naito, Masami Mochizuki, Yukinobu Tohya, Dat Vu, Takeshi Mikami, Eiji Takahashi

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25 Scopus citations

Abstract

Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.

Original languageEnglish (US)
Pages (from-to)609-612
Number of pages4
JournalMicrobiology and Immunology
Volume43
Issue number6
DOIs
StatePublished - Jan 1 1999

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Keywords

  • CPV
  • Cats
  • FPV
  • Vietnam

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Miyazawa, T., Ikeda, Y., Nakamura, K., Naito, R., Mochizuki, M., Tohya, Y., Vu, D., Mikami, T., & Takahashi, E. (1999). Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam. Microbiology and Immunology, 43(6), 609-612. https://doi.org/10.1111/j.1348-0421.1999.tb02447.x