Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Takayuki Miyazawa, Yasuhiro H Ikeda, Kazuya Nakamura, Risako Naito, Masami Mochizuki, Yukinobu Tohya, Dat Vu, Takeshi Mikami, Eiji Takahashi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.

Original languageEnglish (US)
Pages (from-to)609-612
Number of pages4
JournalMicrobiology and Immunology
Volume43
Issue number6
StatePublished - 1999
Externally publishedYes

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Feline Panleukopenia Virus
Vietnam
Felidae
Blood Cells
Cats
Viruses
Neutralizing Antibodies
Kidney
Cell Line
Interleukin-2

Keywords

  • Cats
  • CPV
  • FPV
  • Vietnam

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Miyazawa, T., Ikeda, Y. H., Nakamura, K., Naito, R., Mochizuki, M., Tohya, Y., ... Takahashi, E. (1999). Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam. Microbiology and Immunology, 43(6), 609-612.

Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam. / Miyazawa, Takayuki; Ikeda, Yasuhiro H; Nakamura, Kazuya; Naito, Risako; Mochizuki, Masami; Tohya, Yukinobu; Vu, Dat; Mikami, Takeshi; Takahashi, Eiji.

In: Microbiology and Immunology, Vol. 43, No. 6, 1999, p. 609-612.

Research output: Contribution to journalArticle

Miyazawa, T, Ikeda, YH, Nakamura, K, Naito, R, Mochizuki, M, Tohya, Y, Vu, D, Mikami, T & Takahashi, E 1999, 'Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam', Microbiology and Immunology, vol. 43, no. 6, pp. 609-612.
Miyazawa T, Ikeda YH, Nakamura K, Naito R, Mochizuki M, Tohya Y et al. Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam. Microbiology and Immunology. 1999;43(6):609-612.
Miyazawa, Takayuki ; Ikeda, Yasuhiro H ; Nakamura, Kazuya ; Naito, Risako ; Mochizuki, Masami ; Tohya, Yukinobu ; Vu, Dat ; Mikami, Takeshi ; Takahashi, Eiji. / Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam. In: Microbiology and Immunology. 1999 ; Vol. 43, No. 6. pp. 609-612.
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