Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Takayuki Miyazawa; Yasuhiro Ikeda; Kazuya Nakamura; Risako Naito; Masami Mochizuki; Yukinobu Tohya; Dat Vu; Takeshi Mikami; Eiji Takahashi

doi:10.1111/j.1348-0421.1999.tb02447.x

Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Takayuki Miyazawa, Yasuhiro Ikeda, Kazuya Nakamura, Risako Naito, Masami Mochizuki, Yukinobu Tohya, Dat Vu, Takeshi Mikami, Eiji Takahashi

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.

Original language	English (US)
Pages (from-to)	609-612
Number of pages	4
Journal	Microbiology and Immunology
Volume	43
Issue number	6
DOIs	https://doi.org/10.1111/j.1348-0421.1999.tb02447.x
State	Published - 1999

Keywords

CPV
Cats
FPV
Vietnam

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1999.tb02447.x

Cite this

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title = "Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam",

abstract = "Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.",

keywords = "CPV, Cats, FPV, Vietnam",

author = "Takayuki Miyazawa and Yasuhiro Ikeda and Kazuya Nakamura and Risako Naito and Masami Mochizuki and Yukinobu Tohya and Dat Vu and Takeshi Mikami and Eiji Takahashi",

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T1 - Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

AU - Miyazawa, Takayuki

AU - Ikeda, Yasuhiro

AU - Nakamura, Kazuya

AU - Naito, Risako

AU - Mochizuki, Masami

AU - Tohya, Yukinobu

AU - Vu, Dat

AU - Mikami, Takeshi

AU - Takahashi, Eiji

PY - 1999

Y1 - 1999

N2 - Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.

AB - Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against FPV, indicating that FPV infected PBMCs and was not eliminated from PBMCs even in the presence of VN antibodies in the cats.

KW - CPV

KW - Cats

KW - FPV

KW - Vietnam

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Isolation of feline parvovirus from peripheral blood mononuclear cells of cats in northern Vietnam

Abstract

Keywords

ASJC Scopus subject areas

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