Isolation and mapping of polymorphic microsatellites in cattle

P. Steffen, A. Eggen, G. Stranzinger, R. Fries, A. B. Dietz, J. E. Womack

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Summary A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC‐dA)n‐(dG‐dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR‐analysis of a panel of somatic hybrid lines.

Original languageEnglish (US)
Pages (from-to)121-124
Number of pages4
JournalAnimal Genetics
Volume24
Issue number2
DOIs
StatePublished - Apr 1993

Keywords

  • DNA micro‐satellites
  • bovine
  • gene mapping

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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    Steffen, P., Eggen, A., Stranzinger, G., Fries, R., Dietz, A. B., & Womack, J. E. (1993). Isolation and mapping of polymorphic microsatellites in cattle. Animal Genetics, 24(2), 121-124. https://doi.org/10.1111/j.1365-2052.1993.tb00252.x