Isolation and mapping of polymorphic microsatellites in cattle

P. Steffen; A. Eggen; G. Stranzinger; R. Fries; A. B. Dietz; J. E. Womack

doi:10.1111/j.1365-2052.1993.tb00252.x

Isolation and mapping of polymorphic microsatellites in cattle

P. Steffen, A. Eggen, G. Stranzinger, R. Fries, A. B. Dietz, J. E. Womack

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

125 Scopus citations

Abstract

Summary A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC‐dA)_n‐(dG‐dT)_n oligonucleotide probe for the repeated nucleotide motif (CA)_n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR‐analysis of a panel of somatic hybrid lines.

Original language	English (US)
Pages (from-to)	121-124
Number of pages	4
Journal	Animal Genetics
Volume	24
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2052.1993.tb00252.x
State	Published - Apr 1993

Keywords

DNA micro‐satellites
bovine
gene mapping

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

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10.1111/j.1365-2052.1993.tb00252.x

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abstract = "Summary A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC‐dA)n‐(dG‐dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR‐analysis of a panel of somatic hybrid lines.",

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AU - Steffen, P.

AU - Eggen, A.

AU - Stranzinger, G.

AU - Fries, R.

AU - Dietz, A. B.

AU - Womack, J. E.

PY - 1993/4

Y1 - 1993/4

N2 - Summary A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC‐dA)n‐(dG‐dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR‐analysis of a panel of somatic hybrid lines.

AB - Summary A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC‐dA)n‐(dG‐dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR‐analysis of a panel of somatic hybrid lines.

KW - DNA micro‐satellites

KW - bovine

KW - gene mapping

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Isolation and mapping of polymorphic microsatellites in cattle

Abstract

Keywords

ASJC Scopus subject areas

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