Isolation and analysis of the proteins of plant nuclei: interaction of hormones with nuclear proteins in isolated nuclei of phaseolus vulgaris

Incubation of intact segments or isolated nuclei of bean hypocotyls with IAA-2-¹⁴C and kinetin-8¹⁴C, showed that these hormones associate with the nuclear proteins. Differences in the degree of association of these hormones with the nuclear proteins were found between the isolated nuclei or intact segments. Partially purified nuclei from undifferentiated hook and the more differentiated lower section of bean hypocotyl displayed quantitative and qualitative changes in nuclear proteins due to hormone treatments. Four of the five nuclear protein fractions of each of the tissues were quantitatively reduced by the hormone treatments. The acidic proteins associated with DNA were increased by IAA but not be kinetin. However, most of the protein electrophoretic patterns were qualitatively unchanged by hormone treatment.