TY - JOUR
T1 - Ischemic exercise hyperemia in the human forearm
T2 - Reproducibility and roles of adenosine and nitric oxide
AU - Lopez, Marcos G.
AU - Silva, Bruno M.
AU - Joyner, Michael J.
AU - Casey, Darren P.
N1 - Funding Information:
Acknowledgments We would like to acknowledge Jean Knutson R.N., Karen Krucker R.N., and Shelly Roberts R.N. for assistance in preparing study medications and assisting with arterial lines, Branton Walker for technical assistance throughout these studies, and Pamela Engrav for coordinating this study. Also, we acknowledge the research participants that made this study possible. Grants: This publication was made possible by National Institutes of Health grants HL46493 (MJJ), AR-55819 (DPC) and CTSA RR-024150. The Caywood Professorship via the Mayo Foundation also supported this research. We also acknowledge stipend support from the Coordination for the Improvement of Higher Education Personnel (CAPES).
PY - 2012/6
Y1 - 2012/6
N2 - The roles of local metabolites in reactive and exercise hyperemia remain incompletely understood. Amaximum metabolic stimulus caused by ischemic exercise (IE) could potentially fully activate all vasodilator pathways and limit potential redundancy amongst vasoactive substances. We tested the hypotheses that IE elicits areproducible hyperemic response in the forearm and that adenosine (ADO) and nitric oxide (NO) contribute to this response. In separate protocols, forearm blood flow (FBF) was measured with venous occlusion plethysmography following IE trials consisting of 5 min of ischemia and rhythmic forearm handgrip exercise (performed during last 2 min of ischemia). In protocol 1 (n = 8), FBF was measured after three trials of IE. In protocol 2 (n = 9), subjects performed IE during control (saline), aminophylline (APH; adenosine receptor antagonist), and combined APH/NG-monomethyl-L-arginine (L-NMMA; NOS inhibition) infusions. In protocol 1, coefficients of variation for total (area under the curve) DFBF and DFVC (forearm vascular conductance) following IE were 10.4 ± 1.0% and 14.9 ± 1.0%, respectively. In protocol 2, peak DFBF was similar for saline and APH trials. Peak DFBF for the APH+L-NMMA trial was greater than that of the APH trial (P = 0.03), and peak DFVC was marginally non-significant (P = 0.053). Total DFBF (54.8 ± 3.9, 55.2 ± 5.4, and 60.4 ± 4.8 ml 100 ml-1; P = 0.43) and DFVC (51.4 ± 3.5, 52.1 ± 5.5, and 56.5 ± 5.0 ml 100 ml -1 100 mmHg-1; P = 0.52) were similar for saline, APH, and APH+L-NMMA, respectively. Our data suggest that (1) the hyperemic response to IE is reproducible and (2) inhibition of ADO alone or combined ADO and NO does not blunt the hyperemic response following IE.
AB - The roles of local metabolites in reactive and exercise hyperemia remain incompletely understood. Amaximum metabolic stimulus caused by ischemic exercise (IE) could potentially fully activate all vasodilator pathways and limit potential redundancy amongst vasoactive substances. We tested the hypotheses that IE elicits areproducible hyperemic response in the forearm and that adenosine (ADO) and nitric oxide (NO) contribute to this response. In separate protocols, forearm blood flow (FBF) was measured with venous occlusion plethysmography following IE trials consisting of 5 min of ischemia and rhythmic forearm handgrip exercise (performed during last 2 min of ischemia). In protocol 1 (n = 8), FBF was measured after three trials of IE. In protocol 2 (n = 9), subjects performed IE during control (saline), aminophylline (APH; adenosine receptor antagonist), and combined APH/NG-monomethyl-L-arginine (L-NMMA; NOS inhibition) infusions. In protocol 1, coefficients of variation for total (area under the curve) DFBF and DFVC (forearm vascular conductance) following IE were 10.4 ± 1.0% and 14.9 ± 1.0%, respectively. In protocol 2, peak DFBF was similar for saline and APH trials. Peak DFBF for the APH+L-NMMA trial was greater than that of the APH trial (P = 0.03), and peak DFVC was marginally non-significant (P = 0.053). Total DFBF (54.8 ± 3.9, 55.2 ± 5.4, and 60.4 ± 4.8 ml 100 ml-1; P = 0.43) and DFVC (51.4 ± 3.5, 52.1 ± 5.5, and 56.5 ± 5.0 ml 100 ml -1 100 mmHg-1; P = 0.52) were similar for saline, APH, and APH+L-NMMA, respectively. Our data suggest that (1) the hyperemic response to IE is reproducible and (2) inhibition of ADO alone or combined ADO and NO does not blunt the hyperemic response following IE.
KW - Adenosine
KW - Hyperemia
KW - Ischemia
KW - Nitric oxide
KW - Vasodilation
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U2 - 10.1007/s00421-011-2035-8
DO - 10.1007/s00421-011-2035-8
M3 - Article
C2 - 21947452
AN - SCOPUS:84861529598
SN - 1439-6319
VL - 112
SP - 2065
EP - 2072
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
IS - 6
ER -