Irreversible inhibition of mammalian and yeast S-adenosylmethionine decarboxylase by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine)

Anthony E. Pegg, Cheryl A Conover

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Putrescine activated S-adenosylmethionine decarboxylases from rat liver and yeast are strongly inhibited on incubation with 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine). Inhibition cannot be reversed by dialysis or dilution and occurs more rapidly in the presence of putrescine. Protection against this inactivation is provided by the presence of methylglyoxal bis(guanylhydrazone) which is an analog of the inactivator and is known to be a potent but readily reversible inhibitor of the decarboxylase. Inactivation of S-adenosylmethionine decarboxylase activity by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine) occurs in unfractionated liver homogenates and in rats treated with this compound which may therefore be of value in depressing spermidine synthesis in vivo.

Original languageEnglish (US)
Pages (from-to)766-774
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume69
Issue number3
DOIs
StatePublished - Apr 5 1976
Externally publishedYes

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Adenosylmethionine Decarboxylase
Putrescine
Liver
Yeast
Rats
Mitoguazone
Yeasts
Carboxy-Lyases
Spermidine
Dialysis
Dilution
pimagedine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "Irreversible inhibition of mammalian and yeast S-adenosylmethionine decarboxylase by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine)",
abstract = "Putrescine activated S-adenosylmethionine decarboxylases from rat liver and yeast are strongly inhibited on incubation with 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine). Inhibition cannot be reversed by dialysis or dilution and occurs more rapidly in the presence of putrescine. Protection against this inactivation is provided by the presence of methylglyoxal bis(guanylhydrazone) which is an analog of the inactivator and is known to be a potent but readily reversible inhibitor of the decarboxylase. Inactivation of S-adenosylmethionine decarboxylase activity by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine) occurs in unfractionated liver homogenates and in rats treated with this compound which may therefore be of value in depressing spermidine synthesis in vivo.",
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T1 - Irreversible inhibition of mammalian and yeast S-adenosylmethionine decarboxylase by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine)

AU - Pegg, Anthony E.

AU - Conover, Cheryl A

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N2 - Putrescine activated S-adenosylmethionine decarboxylases from rat liver and yeast are strongly inhibited on incubation with 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine). Inhibition cannot be reversed by dialysis or dilution and occurs more rapidly in the presence of putrescine. Protection against this inactivation is provided by the presence of methylglyoxal bis(guanylhydrazone) which is an analog of the inactivator and is known to be a potent but readily reversible inhibitor of the decarboxylase. Inactivation of S-adenosylmethionine decarboxylase activity by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine) occurs in unfractionated liver homogenates and in rats treated with this compound which may therefore be of value in depressing spermidine synthesis in vivo.

AB - Putrescine activated S-adenosylmethionine decarboxylases from rat liver and yeast are strongly inhibited on incubation with 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine). Inhibition cannot be reversed by dialysis or dilution and occurs more rapidly in the presence of putrescine. Protection against this inactivation is provided by the presence of methylglyoxal bis(guanylhydrazone) which is an analog of the inactivator and is known to be a potent but readily reversible inhibitor of the decarboxylase. Inactivation of S-adenosylmethionine decarboxylase activity by 1,1′-(methylethanediylidenedinitrilo)-bis(3-aminoguanidine) occurs in unfractionated liver homogenates and in rats treated with this compound which may therefore be of value in depressing spermidine synthesis in vivo.

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