Investigations on alkaline protease production with B. subtilis PE-11 immobilized in calcium alginate gel beads

K. Adinarayana, K. V.V.S.N. Bapi Raju, P. Ellaiah

Research output: Contribution to journalArticlepeer-review

Abstract

Bacillus subtilis PE-11 cells were immobilized in calcium alginate and used for the production of alkaline protease. The influence of alginate concentration, different cations, concentration of cation, curing time, bead diameter and nutrient strength on alkaline protease production and stability of biocatalyst were investigated. Repeated batch fermentations of immobilized cells in shake flasks were carried out with the optimized parameters such as; 3% alginate, 0.25 M calcium chloride with 1 h curing time, 3.24 mm bead diameter and 0.75% glucose and 0.75% peptone as nutrients. The results indicated that, a good level of enzyme was maintained for a period of about 9 days. The immobilized cells of B. subtilis PE-11 in calcium alginate are more efficient for the production of alkaline protease with repeated batch fermentation.

Keywords

  • Alkaline protease production
  • Calcium alginate
  • Immobilized cells
  • Repeated batch fermentation

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Investigations on alkaline protease production with B. subtilis PE-11 immobilized in calcium alginate gel beads'. Together they form a unique fingerprint.

Cite this