Investigation of cavernosal smooth muscle dysfunction in low flow priapism using an in vitro model

A. Muneer, S. Cellek, A. Dogan, P. D. Kell, D. J. Ralph, S. Minhas

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The effects of hypoxia (pO2: 50 mmHg), acidosis (pH: 6.9) or glucopenia (absence of glucose) in vitro on the tone of the rabbit corpus cavernosum were investigated. The recovery of smooth muscle contractility following exposure to these conditions was also assessed. Hypoxia, acidosis or glucopenia alone or in combination showed a sustained reduction in the tone. Reperfusion of tissue strips showed complete recovery of smooth muscle tone for all conditions except when hypoxia and glucopenia were combined or when hypoxia, glucopenia and acidosis were used in combination. Incomplete recovery of tone was associated with a significant reduction in tissue ATP concentrations and an increase in the number of TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling)-positive nuclei. This indicates that following reversal of hypoxia, acidosis and glucopenia, failure of conventional α-adrenergic agonists to produce tumescence in low flow priapism is associated with irreversible smooth muscle cell dysfunction, which is linked to ATP reduction and smooth muscle cell death.

Original languageEnglish (US)
Pages (from-to)10-18
Number of pages9
JournalInternational Journal of Impotence Research
Volume17
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Priapism
Smooth Muscle
Acidosis
Smooth Muscle Myocytes
Adenosine Triphosphate
Adrenergic Agonists
DNA Nucleotidylexotransferase
Reperfusion
Cell Death
In Vitro Techniques
Hypoxia
Rabbits
Glucose

Keywords

  • Corpus cavernosum
  • Erectile dysfunction
  • Priapism
  • Rabbit
  • Smooth muscle

ASJC Scopus subject areas

  • Urology

Cite this

Investigation of cavernosal smooth muscle dysfunction in low flow priapism using an in vitro model. / Muneer, A.; Cellek, S.; Dogan, A.; Kell, P. D.; Ralph, D. J.; Minhas, S.

In: International Journal of Impotence Research, Vol. 17, No. 1, 01.2005, p. 10-18.

Research output: Contribution to journalArticle

Muneer, A. ; Cellek, S. ; Dogan, A. ; Kell, P. D. ; Ralph, D. J. ; Minhas, S. / Investigation of cavernosal smooth muscle dysfunction in low flow priapism using an in vitro model. In: International Journal of Impotence Research. 2005 ; Vol. 17, No. 1. pp. 10-18.
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