Interphase fluorescence in situ hybridization studies for the detection of 9q34 deletions in chronic myelogenous leukemia: A practical approach to clinical diagnosis

Patricia Aoun, Michele Wiggins, Diane Pickering, James Foran, Husain Rasheed, Steve Z. Pavletic, Warren Sanger

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Chronic myelogenous leukemia (CML) is characterized by the Philadelphia chromosome (Ph) in more than 90% of cases. Recent studies using fluorescence in situ hybridization (FISH) have shown that in a subset of patients with CML, deletions of 9q34 involving the argininosuccinate synthetase region occur at the time of the Philadelphia translocation and are associated with a poor prognosis. We performed interphase FISH studies in 152 cases of CML using a dual-color, dual-fusion probe system with a third probe directed at 9q34. Cytogenetic studies showed a simple (typical) Ph in 124/152 (82%), a cryptic Ph in 11/152 (7%), and a variant Ph chromosome with a complex translocation in 17/152 (11%) of cases. Interphase FISH studies showed single BCR/ABL fusion patterns in 48/152 (32%) of cases. Deletions of 9q34 were observed in 14% of all the cases and were present in 46% of cases with single BCR/ABL fusion pattern. All the 9q34 deletions occurred in cases with single BCR/ABL fusion signal. However, a single-fusion pattern is not specific for 9q34 deletions, and cases should be routinely screened for the presence of this prognostically significant abnormality by using a third probe directed specifically at 9q34.

Original languageEnglish (US)
Pages (from-to)138-143
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume154
Issue number2
DOIs
StatePublished - Oct 15 2004

    Fingerprint

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this