Interleukin-1 and synovial protein kinase C: Identification of a novel, 35 kDa cytosolic substrate

K. I. Hulkower, R. Sagi-Eisenberg, L. M. Traub, H. I. Georgescu, Christopher H Evans

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have been examining the role of protein kinase C (PKC) in synovial cell activation in response to interleukin-1 (IL-1). Attempts to measure PKC in soluble extracts of synovial fibroblasts by standard techniques failed. Western blotting with anti-PKC antibodies detected only a low level of PKC in synovial cells compared to rat basophilic leukemia cells and crude brain extracts. However, synovial PKC could be detected by measuring the Ca2+- and phospholipid-dependent phosphorylation of endogenous substrates. In this way, a 35 kDa protein was identified as the major endogenous cytosolic substrate for PKC. Treatment of synoviocytes with phorbol myristate acetate (PMA) strongly induced the synthesis of neutral metalloproteinases (NPs) and prostaglandin E2 (PGE2). Both Western blotting and assays based upon phosphorylation of the 35 kDa protein confirmed translocation of PKC from the cytosol in response to PMA. Although IL-1 induced the NPs and PGE2, it did so without detectable translocation of PKC. There thus appear to be PKC-dependent and PKC-independent routes of synovial cell activation. Our data suggest that IL-1 uses the latter.

Original languageEnglish (US)
Pages (from-to)278-281
Number of pages4
JournalAgents and Actions
Volume34
Issue number1-2
StatePublished - 1991
Externally publishedYes

Fingerprint

Interleukin-1
Protein Kinase C
Substrates
Phosphorylation
Metalloproteases
Tetradecanoylphorbol Acetate
Dinoprostone
Western Blotting
Chemical activation
Protein Transport
Complex Mixtures
Fibroblasts
Cytosol
Phospholipids
Leukemia
Rats
Assays
Brain
Proteins
Antibodies

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Toxicology

Cite this

Hulkower, K. I., Sagi-Eisenberg, R., Traub, L. M., Georgescu, H. I., & Evans, C. H. (1991). Interleukin-1 and synovial protein kinase C: Identification of a novel, 35 kDa cytosolic substrate. Agents and Actions, 34(1-2), 278-281.

Interleukin-1 and synovial protein kinase C : Identification of a novel, 35 kDa cytosolic substrate. / Hulkower, K. I.; Sagi-Eisenberg, R.; Traub, L. M.; Georgescu, H. I.; Evans, Christopher H.

In: Agents and Actions, Vol. 34, No. 1-2, 1991, p. 278-281.

Research output: Contribution to journalArticle

Hulkower, KI, Sagi-Eisenberg, R, Traub, LM, Georgescu, HI & Evans, CH 1991, 'Interleukin-1 and synovial protein kinase C: Identification of a novel, 35 kDa cytosolic substrate', Agents and Actions, vol. 34, no. 1-2, pp. 278-281.
Hulkower, K. I. ; Sagi-Eisenberg, R. ; Traub, L. M. ; Georgescu, H. I. ; Evans, Christopher H. / Interleukin-1 and synovial protein kinase C : Identification of a novel, 35 kDa cytosolic substrate. In: Agents and Actions. 1991 ; Vol. 34, No. 1-2. pp. 278-281.
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