Integration of fluorescence imaging with proteomics enables visualization and identification of metallo-proteomes in living cells

Yau Tsz Lai, Ya Yang, Ligang Hu, Tianfan Cheng, Yuen Yan Chang, Mohamad Koohi-Moghadam, Yuchuan Wang, Jiang Xia, Junwen Wang, Hongyan Li, Hongzhe Sun

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Metalloproteins account for nearly one-third of proteins in proteomes. To date, the identification of metalloproteins relies mainly on protein purification and the subsequent characterization of bound metals, which often leads to losses of metal ions bound weakly and transiently. Herein, we developed a strategy to visualize and subsequently identify endogenous metalloproteins and metal-binding proteins in living cells via integration of fluorescence imaging with proteomics. We synthesized a "metal-tunable" fluorescent probe (denoted as Mn+-TRACER) that rapidly enters cells to target proteins with 4-40 fold fluorescence enhancements. By using Ni2+-TRACER as an example, we demonstrate the feasibility of tracking Ni2+-binding proteins in vitro, while cellular small molecules exhibit negligible interference on the labeling. We identified 44 Ni2+-binding proteins from microbes using Helicobacter pylori as a showcase. We further applied Cu2+-TRACER to mammalian cells and found 54 Cu2+-binding proteins. The strategy we report here provides a great opportunity to track various endogenous metallo-proteomes and to mine potential targets of metallodrugs.

Original languageEnglish (US)
Pages (from-to)38-47
Number of pages10
JournalMetallomics
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2017

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Optical Imaging
Metalloproteins
Proteome
Proteomics
Carrier Proteins
Visualization
Metals
Fluorescence
Cells
Proteins
Imaging techniques
Fluorescent Dyes
Helicobacter pylori
Labeling
Purification
Metal ions
Ions
Molecules

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biophysics
  • Biochemistry
  • Biomaterials
  • Metals and Alloys

Cite this

Integration of fluorescence imaging with proteomics enables visualization and identification of metallo-proteomes in living cells. / Lai, Yau Tsz; Yang, Ya; Hu, Ligang; Cheng, Tianfan; Chang, Yuen Yan; Koohi-Moghadam, Mohamad; Wang, Yuchuan; Xia, Jiang; Wang, Junwen; Li, Hongyan; Sun, Hongzhe.

In: Metallomics, Vol. 9, No. 1, 01.01.2017, p. 38-47.

Research output: Contribution to journalArticle

Lai, YT, Yang, Y, Hu, L, Cheng, T, Chang, YY, Koohi-Moghadam, M, Wang, Y, Xia, J, Wang, J, Li, H & Sun, H 2017, 'Integration of fluorescence imaging with proteomics enables visualization and identification of metallo-proteomes in living cells', Metallomics, vol. 9, no. 1, pp. 38-47. https://doi.org/10.1039/c6mt00169f
Lai, Yau Tsz ; Yang, Ya ; Hu, Ligang ; Cheng, Tianfan ; Chang, Yuen Yan ; Koohi-Moghadam, Mohamad ; Wang, Yuchuan ; Xia, Jiang ; Wang, Junwen ; Li, Hongyan ; Sun, Hongzhe. / Integration of fluorescence imaging with proteomics enables visualization and identification of metallo-proteomes in living cells. In: Metallomics. 2017 ; Vol. 9, No. 1. pp. 38-47.
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