Influence of omega-3 fatty acids on skeletal muscle protein metabolism and mitochondrial bioenergetics in older adults

Antigoni Z. Lalia, Surendra Dasari, Matthew M. Robinson, Hinnah Abid, Dawn M. Morse, Katherine A. Klaus, Ian R Lanza

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Omega-3 polyunsaturated fatty acids (n3-PUFA) are recognized for their anti-inflammatory effects and may be beneficial in the context of sarcopenia. We determined the influence of n3-PUFA on muscle mitochondrial physiology and protein metabolism in older adults. Twelve young (18-35 years) and older (65-85 years) men and women were studied at baseline. Older adults were studied again following n3-PUFA supplementation (3.9g/day, 16 weeks). Muscle biopsies were used to evaluate respiratory capacity (high resolution respirometry) and oxidant emissions (spectrofluorometry) in isolated mitochondria. Maximal respiration was significantly lower in older compared to young. n3-PUFA did not change respiration, but significantly reduced oxidant emissions. Participants performed a single bout of resistance exercise, followed by biopsies at 15 and 18 hours post exercise. Several genes involved in muscle protein turnover were significantly altered in older adults at baseline and following exercise, yet muscle protein synthesis was similar between age groups under both conditions. Following n3-PUFA supplementation, mixed muscle, mitochondrial, and sarcoplasmic protein synthesis rates were increased in older adults before exercise. n3-PUFA increased post-exercise mitochondrial and myofibrillar protein synthesis in older adults. These results demonstrate that n3-PUFA reduce mitochondrial oxidant emissions, increase postabsorptive muscle protein synthesis, and enhance anabolic responses to exercise in older adults.

Original languageEnglish (US)
Pages (from-to)1096-1129
Number of pages34
JournalAging
Volume9
Issue number4
DOIs
StatePublished - 2017

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Muscle Proteins
Omega-3 Fatty Acids
Energy Metabolism
Skeletal Muscle
Exercise
Mitochondrial Proteins
Oxidants
Respiration
Sarcopenia
Biopsy
Muscles
Fluorescence Spectrometry
Unsaturated Fatty Acids
Mitochondria
Anti-Inflammatory Agents
Age Groups

Keywords

  • DHA
  • EPA
  • Mitochondria
  • N3-PUFA
  • Protein synthesis
  • Sarcopenia

ASJC Scopus subject areas

  • Aging
  • Cell Biology

Cite this

Influence of omega-3 fatty acids on skeletal muscle protein metabolism and mitochondrial bioenergetics in older adults. / Lalia, Antigoni Z.; Dasari, Surendra; Robinson, Matthew M.; Abid, Hinnah; Morse, Dawn M.; Klaus, Katherine A.; Lanza, Ian R.

In: Aging, Vol. 9, No. 4, 2017, p. 1096-1129.

Research output: Contribution to journalArticle

Lalia, Antigoni Z. ; Dasari, Surendra ; Robinson, Matthew M. ; Abid, Hinnah ; Morse, Dawn M. ; Klaus, Katherine A. ; Lanza, Ian R. / Influence of omega-3 fatty acids on skeletal muscle protein metabolism and mitochondrial bioenergetics in older adults. In: Aging. 2017 ; Vol. 9, No. 4. pp. 1096-1129.
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