Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins

J. A. Miskimen, D. P. Guertin, D. P. Fan, C. S. David

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Spleen cells from a number of H-2 congenic mice were tested for their proliferative and/or cytolytic responses to partially purified products from CNBr cleavage of Sendai virus. In response to in vitro stimulation of Sendai-primed spleen cells with virus-infected cells, Sendai virus-specific cytolytic T lymphocytes restricted to H-2K(b), K(d), K(k), K(a), and D(d) were generated. Of these, H-2K(b)- and K(d)-, but not H-2K(k)-, K(a)-, and D(d)-, restricted cytolytic T cells were generated after stimulation with partially purified Sendai peptides. H-2D(d)-restmicted, virus-specific cytotoxicity was generated in response to unseparated products from CNBr cleavage of Sendai virus. Thus, the pool of partially purified peptides contains some but not all of the antigenic determinants that can stimulate a cytolytic response. These results suggest that each determinant on Sendai viral proteins may stimulate cytolytic T lymphocytes restricted to only a subset of the H-2K and D alleles which can be recognized by the sum of cytolytic T cells generated in response to all of the antigenic determinants on Sendai viral proteins. Thus, H-2K and D genes function as immune response genes regulating the cytolytic response to partially purified peptides and, by extension, possibly to individual antigenic determinants on Sendai viral proteins. Also, spleen cells from three mice differing only in the H-2I-S regions show differences in their proliferative responses to Sendai antigens. Only spleen cells from mice with the k haplotype in IA-IJ proliferate in response to partially purified peptides, whereas spleen cells from all three mice proliferate when virus is added to the cultures. These proliferating cells are sensitive to treatment with anti-Thy-1.2 and C, suggesting that immune response genes regulating T cell proliferative responses to individual viral determinants may also exist.

Original languageEnglish (US)
Pages (from-to)1522-1528
Number of pages7
JournalJournal of Immunology
Volume128
Issue number4
StatePublished - 1982
Externally publishedYes

Fingerprint

Viral Proteins
T-Lymphocytes
Spleen
Sendai virus
Peptides
Genes
Epitopes
Viruses
Congenic Mice
Haplotypes
Cell Count
Alleles
Antigens
H-2K(K) antigen

ASJC Scopus subject areas

  • Immunology

Cite this

Miskimen, J. A., Guertin, D. P., Fan, D. P., & David, C. S. (1982). Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins. Journal of Immunology, 128(4), 1522-1528.

Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins. / Miskimen, J. A.; Guertin, D. P.; Fan, D. P.; David, C. S.

In: Journal of Immunology, Vol. 128, No. 4, 1982, p. 1522-1528.

Research output: Contribution to journalArticle

Miskimen, JA, Guertin, DP, Fan, DP & David, CS 1982, 'Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins', Journal of Immunology, vol. 128, no. 4, pp. 1522-1528.
Miskimen, J. A. ; Guertin, D. P. ; Fan, D. P. ; David, C. S. / Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins. In: Journal of Immunology. 1982 ; Vol. 128, No. 4. pp. 1522-1528.
@article{0d1766cff8b142fba083b4f730b75366,
title = "Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins",
abstract = "Spleen cells from a number of H-2 congenic mice were tested for their proliferative and/or cytolytic responses to partially purified products from CNBr cleavage of Sendai virus. In response to in vitro stimulation of Sendai-primed spleen cells with virus-infected cells, Sendai virus-specific cytolytic T lymphocytes restricted to H-2K(b), K(d), K(k), K(a), and D(d) were generated. Of these, H-2K(b)- and K(d)-, but not H-2K(k)-, K(a)-, and D(d)-, restricted cytolytic T cells were generated after stimulation with partially purified Sendai peptides. H-2D(d)-restmicted, virus-specific cytotoxicity was generated in response to unseparated products from CNBr cleavage of Sendai virus. Thus, the pool of partially purified peptides contains some but not all of the antigenic determinants that can stimulate a cytolytic response. These results suggest that each determinant on Sendai viral proteins may stimulate cytolytic T lymphocytes restricted to only a subset of the H-2K and D alleles which can be recognized by the sum of cytolytic T cells generated in response to all of the antigenic determinants on Sendai viral proteins. Thus, H-2K and D genes function as immune response genes regulating the cytolytic response to partially purified peptides and, by extension, possibly to individual antigenic determinants on Sendai viral proteins. Also, spleen cells from three mice differing only in the H-2I-S regions show differences in their proliferative responses to Sendai antigens. Only spleen cells from mice with the k haplotype in IA-IJ proliferate in response to partially purified peptides, whereas spleen cells from all three mice proliferate when virus is added to the cultures. These proliferating cells are sensitive to treatment with anti-Thy-1.2 and C, suggesting that immune response genes regulating T cell proliferative responses to individual viral determinants may also exist.",
author = "Miskimen, {J. A.} and Guertin, {D. P.} and Fan, {D. P.} and David, {C. S.}",
year = "1982",
language = "English (US)",
volume = "128",
pages = "1522--1528",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Influence of H-2-linked genes on T cell proliferative and cytolytic responses to peptides of Sendai viral proteins

AU - Miskimen, J. A.

AU - Guertin, D. P.

AU - Fan, D. P.

AU - David, C. S.

PY - 1982

Y1 - 1982

N2 - Spleen cells from a number of H-2 congenic mice were tested for their proliferative and/or cytolytic responses to partially purified products from CNBr cleavage of Sendai virus. In response to in vitro stimulation of Sendai-primed spleen cells with virus-infected cells, Sendai virus-specific cytolytic T lymphocytes restricted to H-2K(b), K(d), K(k), K(a), and D(d) were generated. Of these, H-2K(b)- and K(d)-, but not H-2K(k)-, K(a)-, and D(d)-, restricted cytolytic T cells were generated after stimulation with partially purified Sendai peptides. H-2D(d)-restmicted, virus-specific cytotoxicity was generated in response to unseparated products from CNBr cleavage of Sendai virus. Thus, the pool of partially purified peptides contains some but not all of the antigenic determinants that can stimulate a cytolytic response. These results suggest that each determinant on Sendai viral proteins may stimulate cytolytic T lymphocytes restricted to only a subset of the H-2K and D alleles which can be recognized by the sum of cytolytic T cells generated in response to all of the antigenic determinants on Sendai viral proteins. Thus, H-2K and D genes function as immune response genes regulating the cytolytic response to partially purified peptides and, by extension, possibly to individual antigenic determinants on Sendai viral proteins. Also, spleen cells from three mice differing only in the H-2I-S regions show differences in their proliferative responses to Sendai antigens. Only spleen cells from mice with the k haplotype in IA-IJ proliferate in response to partially purified peptides, whereas spleen cells from all three mice proliferate when virus is added to the cultures. These proliferating cells are sensitive to treatment with anti-Thy-1.2 and C, suggesting that immune response genes regulating T cell proliferative responses to individual viral determinants may also exist.

AB - Spleen cells from a number of H-2 congenic mice were tested for their proliferative and/or cytolytic responses to partially purified products from CNBr cleavage of Sendai virus. In response to in vitro stimulation of Sendai-primed spleen cells with virus-infected cells, Sendai virus-specific cytolytic T lymphocytes restricted to H-2K(b), K(d), K(k), K(a), and D(d) were generated. Of these, H-2K(b)- and K(d)-, but not H-2K(k)-, K(a)-, and D(d)-, restricted cytolytic T cells were generated after stimulation with partially purified Sendai peptides. H-2D(d)-restmicted, virus-specific cytotoxicity was generated in response to unseparated products from CNBr cleavage of Sendai virus. Thus, the pool of partially purified peptides contains some but not all of the antigenic determinants that can stimulate a cytolytic response. These results suggest that each determinant on Sendai viral proteins may stimulate cytolytic T lymphocytes restricted to only a subset of the H-2K and D alleles which can be recognized by the sum of cytolytic T cells generated in response to all of the antigenic determinants on Sendai viral proteins. Thus, H-2K and D genes function as immune response genes regulating the cytolytic response to partially purified peptides and, by extension, possibly to individual antigenic determinants on Sendai viral proteins. Also, spleen cells from three mice differing only in the H-2I-S regions show differences in their proliferative responses to Sendai antigens. Only spleen cells from mice with the k haplotype in IA-IJ proliferate in response to partially purified peptides, whereas spleen cells from all three mice proliferate when virus is added to the cultures. These proliferating cells are sensitive to treatment with anti-Thy-1.2 and C, suggesting that immune response genes regulating T cell proliferative responses to individual viral determinants may also exist.

UR - http://www.scopus.com/inward/record.url?scp=0020062882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020062882&partnerID=8YFLogxK

M3 - Article

C2 - 6174603

AN - SCOPUS:0020062882

VL - 128

SP - 1522

EP - 1528

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -