Abstract
Abstract: Synthesis of methionine5‐enkephalin by intact cells of murine neuroblastoma clone N1E‐115 has been demonstrated both immunocytochemically and biochemically. In addition, N1E‐115 cells possess homogeneous enkephalin (δ) receptors which inhibit prostaglandin E1‐ induced intracellular cyclic AMP formation. An assay was developed for measuring de novo synthesis of methionine5‐enkephalin by pulsing cells in culture with radioactive methionine and isolating this pentapeptide to radiochemical purity by a procedure that included immunoaffinity chromatography specific for oxidized methionine5‐enkephalin. This assay indicated that production of radiolabeled‐methionine5‐enkephalin was increased upon lengthy exposure of intact N1E‐115 cells in the late logarithmic phase of growth to a nonproteolyz‐able analog of methionine5‐enkephalin. This increase in synthesis of intracellular methionine5‐enkephalin relative to control cells was prevented by prior incubation of the clone with naloxone, indicating that the response was mediated by the δ receptor.
Original language | English (US) |
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Pages (from-to) | 922-928 |
Number of pages | 7 |
Journal | Journal of neurochemistry |
Volume | 44 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1985 |
Keywords
- Methionine‐enkephalin
- Neuroblastoma
- Opioid
- δReceptors
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Biochemistry