TY - JOUR
T1 - Inflammatory changes in the central nervous system are associated with behavioral impairment in Plasmodium berghei (strain ANKA)-infected mice
AU - Lacerda-Queiroz, Norinne
AU - Rodrigues, David Henrique
AU - Vilela, Márcia Carvalho
AU - Miranda, Aline Silva de
AU - Amaral, Débora Cristina Guerra
AU - Camargos, Elizabeth Ribeiro da Silva
AU - Carvalho, Leonardo José de Moura
AU - Howe, Charles L.
AU - Teixeira, Mauro Martins
AU - Teixeira, Antônio Lúcio
N1 - Funding Information:
The authors thank Carlos Henrique da Silva and Catherine Kelly Martins for their technical assistance. This work was financially supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig) , Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) , Rede Instituto Brasileiro de Neurociência (Rede IBN-Net) , Brazil. LJMC is recipient of NIH Grants R01-HL087290 (NHLBI) and R01-AI082610 (NIAID) .
PY - 2010/7
Y1 - 2010/7
N2 - Experimental cerebral malaria is a neuroinflammatory condition that results from the host immune response to the parasite. Using intravital microscopy, we investigated leukocyte recruitment in the brain microcirculation and the temporal relationship of this process to the behavioral changes observed in Plasmodium berghei (strain ANKA)-infected C57Bl/6 mice. We found that leukocyte recruitment was increased from day 5 post-infection (p.i.) onwards. Histopathological changes and increased levels of inflammatory cytokines in the brain were also observed. Behavioral performance evaluated by the SHIRPA protocol showed functional impairment from day 6 p.i. onwards. Thus, early leukocyte migration into the brain and associated inflammatory changes may be involved in neurological impairment in parasite-infected C57Bl/6 mice.
AB - Experimental cerebral malaria is a neuroinflammatory condition that results from the host immune response to the parasite. Using intravital microscopy, we investigated leukocyte recruitment in the brain microcirculation and the temporal relationship of this process to the behavioral changes observed in Plasmodium berghei (strain ANKA)-infected C57Bl/6 mice. We found that leukocyte recruitment was increased from day 5 post-infection (p.i.) onwards. Histopathological changes and increased levels of inflammatory cytokines in the brain were also observed. Behavioral performance evaluated by the SHIRPA protocol showed functional impairment from day 6 p.i. onwards. Thus, early leukocyte migration into the brain and associated inflammatory changes may be involved in neurological impairment in parasite-infected C57Bl/6 mice.
KW - Behavior
KW - Cerebral malaria
KW - Cytokines
KW - Intravital microscopy
KW - Leukocyte recruitment
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U2 - 10.1016/j.exppara.2010.02.002
DO - 10.1016/j.exppara.2010.02.002
M3 - Article
C2 - 20138873
AN - SCOPUS:77952581022
SN - 0014-4894
VL - 125
SP - 271
EP - 278
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -