Inducing and expanding regulatory T cell populations by foreign antigen

Karsten Kretschmer, Irina Apostolou, Daniel Hawiger, Khashayarsha Khazaie, Michel C. Nussenzweig, Harald von Boehmer

Research output: Contribution to journalArticle

927 Citations (Scopus)

Abstract

Evidence suggests that regulatory T cells expressing the transcription factor Foxp3 develop extrathymically and intrathymically. Mechanisms of extrathymic induction require further scrutiny, especially as proliferation and/or phenotypic changes of preexisting suppressor cells must be distinguished from true de novo generation. Here we report the conversion of truly naive CD4+ T cells into suppressor cells expressing Foxp3 by targeting of peptide-agonist ligands to dendritic cells and by analysis of Foxp3 expression at the level of single cells. We show that conversion was achieved by minute antigen doses with suboptimal dendritic cell activation. The addition of transforming growth factor-β or the absence of interleukin 2 production, which reduces proliferation, enhanced the conversion rate. In addition, regulatory T cell populations induced in subimmunogenic conditions could subsequently be expanded by delivery of antigen in immunogenic conditions. The extrathymic generation and proliferation of regulatory T cells may contribute to self-tolerance as well as the poor immunogenicity of tumors and may be exploited clinically to prevent or reverse unwanted immunity.

Original languageEnglish (US)
Pages (from-to)1219-1227
Number of pages9
JournalNature Immunology
Volume6
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

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Regulatory T-Lymphocytes
Antigens
Dendritic Cells
Population
Self Tolerance
Transforming Growth Factors
Interleukin-2
Immunity
Transcription Factors
Ligands
T-Lymphocytes
Peptides
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Kretschmer, K., Apostolou, I., Hawiger, D., Khazaie, K., Nussenzweig, M. C., & von Boehmer, H. (2005). Inducing and expanding regulatory T cell populations by foreign antigen. Nature Immunology, 6(12), 1219-1227. https://doi.org/10.1038/ni1265

Inducing and expanding regulatory T cell populations by foreign antigen. / Kretschmer, Karsten; Apostolou, Irina; Hawiger, Daniel; Khazaie, Khashayarsha; Nussenzweig, Michel C.; von Boehmer, Harald.

In: Nature Immunology, Vol. 6, No. 12, 12.2005, p. 1219-1227.

Research output: Contribution to journalArticle

Kretschmer, K, Apostolou, I, Hawiger, D, Khazaie, K, Nussenzweig, MC & von Boehmer, H 2005, 'Inducing and expanding regulatory T cell populations by foreign antigen', Nature Immunology, vol. 6, no. 12, pp. 1219-1227. https://doi.org/10.1038/ni1265
Kretschmer K, Apostolou I, Hawiger D, Khazaie K, Nussenzweig MC, von Boehmer H. Inducing and expanding regulatory T cell populations by foreign antigen. Nature Immunology. 2005 Dec;6(12):1219-1227. https://doi.org/10.1038/ni1265
Kretschmer, Karsten ; Apostolou, Irina ; Hawiger, Daniel ; Khazaie, Khashayarsha ; Nussenzweig, Michel C. ; von Boehmer, Harald. / Inducing and expanding regulatory T cell populations by foreign antigen. In: Nature Immunology. 2005 ; Vol. 6, No. 12. pp. 1219-1227.
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