Increased levels of circulating nitrates and impaired endothelium- mediated vasodilation suggest multiple roles of nitric oxide during acute rejection of pulmonary allografts

L. Wiklund, D. H. Lewis, P. O. Sjoquist, F. Nilsson, H. Tazelaar, Virginia M Miller, C. G A McGregor

Research output: Contribution to journalArticle

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Abstract

Experiments were designed to determine whether changes in pulmonary artery function could be reduced by treatment with a lipid peroxidation inhibitor (H 290/51) during acute rejection of pulmonary allografts. Single lung transplantation was performed in three groups of dogs: group 1 was maintained on immunosuppression for 8 days after operation (immunosuppressed, n = 5); in group 2, immunosuppression was discontinued on postoperative day 5, so that rejection occurred on postoperative day 8 (rejecting, n = 6); in group 3, immunosuppression was discontinued after 5 days, and the lipid peroxidation inhibitor H 290/51 (25 mg/kg) was given perorally for 3 days (rejecting + H 290/51, n = 6). Plasma nitric oxide (NO(x)) was measured by use of chemiluminescence. On postoperative day 8 rejection was observed in groups 2 and 3. Contractions to angiotensin I and endothelium-dependent relaxations to adenosine diphosphate were reduced in pulmonary arteries from rejecting lungs. Responses of rings from dogs treated with H 290/51 were similar to those from rejecting lungs. Rejection did not alter relaxations to exogenous nitric oxide. However, plasma levels of NO(x) increased significantly during rejection independently of treatment with H 290/51. Results of this study confirm that endothelium-dependent relaxation of pulmonary arteries is reduced during acute rejection of lung allografts. The result extends these observations to suggest that treatment with a lipid peroxidation inhibitor neither protects the pulmonary artery function nor affects levels of circulating NO(x). Therefore mechanisms other than lipid peroxidation participate in vascular changes associated with allograft rejection.

Original languageEnglish (US)
Pages (from-to)517-523
Number of pages7
JournalJournal of Heart and Lung Transplantation
Volume16
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Vasodilation
Nitrates
Endothelium
Allografts
Nitric Oxide
Pulmonary Artery
Lung
Immunosuppression
Dogs
Angiotensin I
Lung Transplantation
Luminescence
Adenosine Diphosphate
Lipid Peroxidation
Blood Vessels
Therapeutics
H290-51
lipid peroxidation inhibitor

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Transplantation

Cite this

Increased levels of circulating nitrates and impaired endothelium- mediated vasodilation suggest multiple roles of nitric oxide during acute rejection of pulmonary allografts. / Wiklund, L.; Lewis, D. H.; Sjoquist, P. O.; Nilsson, F.; Tazelaar, H.; Miller, Virginia M; McGregor, C. G A.

In: Journal of Heart and Lung Transplantation, Vol. 16, No. 5, 1997, p. 517-523.

Research output: Contribution to journalArticle

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