TY - JOUR
T1 - Increased iron and ferritin content of sputum from patients with cystic fibrosis or chronic bronchitis
AU - Stites, Steve W.
AU - Walters, Brian
AU - O'Brien-Ladner, Amy R.
AU - Bailey, Kirstin
AU - Wesselius, Lewis J.
N1 - Funding Information:
Supported by the Department of Veterans Affairs, Research Service.
PY - 1998
Y1 - 1998
N2 - Purpose: Extracellular free iron, or iron bound to ferritin, may promote oxidative injury and bacterial growth in airways of patients with chronic airway inflammation due to cystic fibrosis (CF) or chronic bronchitis (CB). In this study, we assessed sputum content of total iron, ferritin, and transferrin in patients with CF or CB as well as sputum from normal subjects with acute airway inflammation caused by viral upper respiratory tract infections (URTIs). Methods: Spontaneously produced sputum was obtained from 33 subjects, including 10 subjects with CF, 18 subjects with CB (10 acute exacerbations, 8 with stable CB), and 5 subjects with URTIs (control subjects). After lysing and dilution, total iron concentrations were determined by controlled coulometry, ferritin was measured by radioimmunoassay, and transferrin was measured by enzyme-linked immunosorbent assay. Results: Iron was not present in detectable amounts in control sputums, but ferritin was present (6±2 ng/mg protein, mean±SE), as was transferrin (2.37±0.44 μg/mg). Compared with control subjects, concentrations of iron in sputum were increased in patient groups with higher amounts in CF patients (242±47 ng/mg, p<0.01) than CB patients with acute exacerbations or patients with stable CB (98±50 and 42±12 ng/mg, p <0.05 for both). Ferritin content of sputum was also increased in each group, with CF patients (113±22 ng/mg, p<0.001) higher than CB patients (acute, 45±10 ng/mg; stable, 87±24 ng/mg; p<0.01 for both). Compared with control subjects, sputum transferrin was decreased in CF patients (1.09±0.40 μg/mg, p<0.05), but not CB patients. Conclusions: These findings indicate there are increased airway concentrations of total iron and ferritin-bound iron in patients with CB and, to a greater extent, in patients with CF. Particularly in CF patients who also demonstrated decreased airway concentrations of transferrin, ferritin-bound iron in airways may promote oxidative injury and enhance bacterial growth.
AB - Purpose: Extracellular free iron, or iron bound to ferritin, may promote oxidative injury and bacterial growth in airways of patients with chronic airway inflammation due to cystic fibrosis (CF) or chronic bronchitis (CB). In this study, we assessed sputum content of total iron, ferritin, and transferrin in patients with CF or CB as well as sputum from normal subjects with acute airway inflammation caused by viral upper respiratory tract infections (URTIs). Methods: Spontaneously produced sputum was obtained from 33 subjects, including 10 subjects with CF, 18 subjects with CB (10 acute exacerbations, 8 with stable CB), and 5 subjects with URTIs (control subjects). After lysing and dilution, total iron concentrations were determined by controlled coulometry, ferritin was measured by radioimmunoassay, and transferrin was measured by enzyme-linked immunosorbent assay. Results: Iron was not present in detectable amounts in control sputums, but ferritin was present (6±2 ng/mg protein, mean±SE), as was transferrin (2.37±0.44 μg/mg). Compared with control subjects, concentrations of iron in sputum were increased in patient groups with higher amounts in CF patients (242±47 ng/mg, p<0.01) than CB patients with acute exacerbations or patients with stable CB (98±50 and 42±12 ng/mg, p <0.05 for both). Ferritin content of sputum was also increased in each group, with CF patients (113±22 ng/mg, p<0.001) higher than CB patients (acute, 45±10 ng/mg; stable, 87±24 ng/mg; p<0.01 for both). Compared with control subjects, sputum transferrin was decreased in CF patients (1.09±0.40 μg/mg, p<0.05), but not CB patients. Conclusions: These findings indicate there are increased airway concentrations of total iron and ferritin-bound iron in patients with CB and, to a greater extent, in patients with CF. Particularly in CF patients who also demonstrated decreased airway concentrations of transferrin, ferritin-bound iron in airways may promote oxidative injury and enhance bacterial growth.
KW - Chronic bronchitis
KW - Cystic fibrosis
KW - Ferritin
KW - Iron
KW - Sputum
UR - http://www.scopus.com/inward/record.url?scp=0031687134&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031687134&partnerID=8YFLogxK
U2 - 10.1378/chest.114.3.814
DO - 10.1378/chest.114.3.814
M3 - Article
C2 - 9743172
AN - SCOPUS:0031687134
SN - 0012-3692
VL - 114
SP - 814
EP - 819
JO - Chest
JF - Chest
IS - 3
ER -