In vivo staphylococcal superantigen-driven polyclonal Ig responses in mice: Dependence upon CD4+ cells and human MHC class II

William Stohl, Dong Xu, Song Zang, Kyung S. Kim, Lily Li, Julie A. Hanson, Stephen A. Stohlman, Chella S. David, Chaim O. Jacob

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Staphylococcal enterotoxin (SE) B and seven other staphylococcal superantigens (SAg), despite promoting vigorous Ig production in human peripheral blood mononuclear cell cultures, are exceedingly poor at eliciting Ig responses in cultures of spleen cells from C57BL/10J (B10) or C3H/HeJ mice. In contrast, SEB elicits Ig responses in cultures of spleen cells from human MHC class II-transgenic mice. Whereas i.p. administration of SEB (0.2-20 μg) to non-transgenic B10 mice elicits very weak in vivo Ig responses, identical treatment of CD4+ cell-intact (but not CD4+ cell-depleted) human MHC class II-transgenic mice elicits dramatic increases in both splenic Ig-secreting cells and serum Ig levels. Over a 2-week period, the SEB-induced in vivo Ig responses peak and then plateau or fall in association with a preferential increase in splenic CD8+ cells. Nevertheless, in vivo depletion of CD8+ cells has no sustained effect on SEB-driven Ig responses. Taken together, these observations demonstrate that the effects of SAg on in vivo humoral immune responses are highly CD4+ cell dependent, are substantially CD8+ cell independent and can be successfully investigated using human MHC class II-transgenic mice. This model system may be useful in investigating the polyclonally activating effects of microbial products (prototypic environmental insults) on the development of systemic autoimmunity.

Original languageEnglish (US)
Pages (from-to)1291-1300
Number of pages10
JournalInternational Immunology
Volume13
Issue number10
StatePublished - 2001

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Superantigens
Transgenic Mice
Cell Culture Techniques
Spleen
Inbred C3H Mouse
Humoral Immunity
Autoimmunity
Blood Cells
Serum

Keywords

  • CD8 cells
  • Staphylococcal enterotoxin B
  • Transgenic/knockout mice

ASJC Scopus subject areas

  • Immunology

Cite this

Stohl, W., Xu, D., Zang, S., Kim, K. S., Li, L., Hanson, J. A., ... Jacob, C. O. (2001). In vivo staphylococcal superantigen-driven polyclonal Ig responses in mice: Dependence upon CD4+ cells and human MHC class II. International Immunology, 13(10), 1291-1300.

In vivo staphylococcal superantigen-driven polyclonal Ig responses in mice : Dependence upon CD4+ cells and human MHC class II. / Stohl, William; Xu, Dong; Zang, Song; Kim, Kyung S.; Li, Lily; Hanson, Julie A.; Stohlman, Stephen A.; David, Chella S.; Jacob, Chaim O.

In: International Immunology, Vol. 13, No. 10, 2001, p. 1291-1300.

Research output: Contribution to journalArticle

Stohl, W, Xu, D, Zang, S, Kim, KS, Li, L, Hanson, JA, Stohlman, SA, David, CS & Jacob, CO 2001, 'In vivo staphylococcal superantigen-driven polyclonal Ig responses in mice: Dependence upon CD4+ cells and human MHC class II', International Immunology, vol. 13, no. 10, pp. 1291-1300.
Stohl, William ; Xu, Dong ; Zang, Song ; Kim, Kyung S. ; Li, Lily ; Hanson, Julie A. ; Stohlman, Stephen A. ; David, Chella S. ; Jacob, Chaim O. / In vivo staphylococcal superantigen-driven polyclonal Ig responses in mice : Dependence upon CD4+ cells and human MHC class II. In: International Immunology. 2001 ; Vol. 13, No. 10. pp. 1291-1300.
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