In vivo quantitation of intratumoral radioisotope uptake using micro-single photon emission computed tomography/computed tomography

Stephanie K Carlson, Kelly L. Classic, Elizabeth M. Hadac, Claire E. Bender, Bradley J. Kemp, Val Lowe, Tanya L. Hoskin, Stephen J Russell

Research output: Contribution to journalArticle

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Abstract

Purpose: This study was undertaken to determine the ability of micro-single photon emission computed tomography (micro-SPECT)/computed tomography (CT) to accurately quantitate intratumoral radioisotope uptake in vivo and to compare these measurements with planar imaging and micro-SPECT imaging alone. Procedures: Human pancreatic cancer xenografts were established in 10 mice. Intratumoral radioisotope uptake was achieved via intratumoral injection of an attenuated measles virus vector expressing the NIS gene (MV-NIS). On various days after MV-NIS injection, 123I planar and micro-SPECT/CT imaging was performed. Tumor activity was determined by dose calibrator measurements and region-of-interest (ROI) image analysis. Agreement and reproducibility of tumor activity measurements were assessed by Bland-Altman plots and Lin's concordance correlation coefficient (CCC). Results: Intratumoral radioisotope uptake was detected in all mice. Scatterplots demonstrate strong agreement (CCC=0.93) between micro-SPECT/ CT ROI image analysis and dose calibrator tumor activity measurements. The differences between dose calibrator activity measurements and those obtained with ROI image analysis of micro-SPECT alone and planar imaging are less accurate and more variable (CCC=0.84 and 0.78, respectively). Conclusions: Micro-SPECT/CT can be used to accurately quantify intratumoral radioisotope uptake in vivo and is more reliable than planar or micro-SPECT imaging alone.

Original languageEnglish (US)
Pages (from-to)324-332
Number of pages9
JournalMolecular Imaging and Biology
Volume8
Issue number6
DOIs
StatePublished - Nov 2006

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Single-Photon Emission-Computed Tomography
Radioisotopes
Neoplasms
Measles virus
Injections
Pancreatic Neoplasms
Heterografts
Tomography
Single Photon Emission Computed Tomography Computed Tomography
Genes

Keywords

  • Measles virus
  • Mice
  • Micro-SPECT
  • Molecular imaging
  • Quantitation
  • Reporter gene
  • Sodium iodide symporter (NIS) gene
  • SPECT

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Radiology Nuclear Medicine and imaging

Cite this

In vivo quantitation of intratumoral radioisotope uptake using micro-single photon emission computed tomography/computed tomography. / Carlson, Stephanie K; Classic, Kelly L.; Hadac, Elizabeth M.; Bender, Claire E.; Kemp, Bradley J.; Lowe, Val; Hoskin, Tanya L.; Russell, Stephen J.

In: Molecular Imaging and Biology, Vol. 8, No. 6, 11.2006, p. 324-332.

Research output: Contribution to journalArticle

Carlson, Stephanie K ; Classic, Kelly L. ; Hadac, Elizabeth M. ; Bender, Claire E. ; Kemp, Bradley J. ; Lowe, Val ; Hoskin, Tanya L. ; Russell, Stephen J. / In vivo quantitation of intratumoral radioisotope uptake using micro-single photon emission computed tomography/computed tomography. In: Molecular Imaging and Biology. 2006 ; Vol. 8, No. 6. pp. 324-332.
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