@article{d04c2eed3c4540b7b940a988006bdf87,
title = "In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species",
abstract = "Kiechle et al. present two transgenic mouse models that allow direct quantitative and spatial detection of α-synuclein (α-syn) oligomers in vivo. They demonstrate α-syn aggregation at the synapse, age-dependent accumulation of 8–16-mer α-syn oligomer species, and trans-cellular oligomer spreading from forebrain to hindbrain neurons.",
keywords = "Parkinson's disease, aging, alpha-synuclein, neurodegeneration, oligomerization, progressive phenotype, protein-fragment complementation, spreading, synaptic oligomers, transgenic mouse model",
author = "Martin Kiechle and {von Einem}, Bjoern and Lennart H{\"o}fs and Patrizia Voehringer and Veselin Grozdanov and Daniel Markx and Rosanna Parlato and Diana Wiesner and Benjamin Mayer and Olena Sakk and Bernd Baumann and Soeren Lukassen and Birgit Liss and Ekici, {Arif B.} and Ludolph, {Albert C.} and Paul Walther and Boris Ferger and McLean, {Pamela J.} and Falkenburger, {Bj{\"o}rn H.} and Weishaupt, {Jochen H.} and Danzer, {Karin M.}",
note = "Funding Information: We thank T. Christ, R. Bueck, and A. Jesionek for excellent work. We thank F. Roselli for ISH assistance. We also thank the Core Facility for transgenic animals and for Laser Microscopy at Ulm University. Moreover, we are grateful to R. Marienfeld from CCCU for the opportunity to work with the Zeiss PALM MicroBeam system. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) Emmy Noether Research Group DA 1657/2-1 and GRK 1789 (CEMMA), funds from the Junior Professorship Program Baden-Wuerttemberg, as well as FWF SFB F-44 and DFG LI-1745/1. M.K. and K.M.D. designed the project. O.S. B.v.E. and B.B. established transgenic mouse lines. M.K. performed experiments and analyzed the data. S.L. and A.B.E. performed RNA-seq analysis. M.K. and V.G. analyzed the results with IPA. L.H. B.H.F. R.P. and B.L. performed stereology of TH neurons, and R.P. supported vibratome sectioning. P.V. and B.F. performed HPLC analysis. D.M. assisted with SEC assays. D.W. helped with mouse experiments. B.M. performed statistical analysis. V.G. P.W. B.B. A.C.L. P.J.M. and J.H.W. provided intellectual input. M.K. J.H.W. and K.M.D. wrote the manuscript. All authors reviewed and approved the manuscript. The authors declare no competing interests. Funding Information: We thank T. Christ, R. Bueck, and A. Jesionek for excellent work. We thank F. Roselli for ISH assistance. We also thank the Core Facility for transgenic animals and for Laser Microscopy at Ulm University. Moreover, we are grateful to R. Marienfeld from CCCU for the opportunity to work with the Zeiss PALM MicroBeam system. This work was supported by the Deutsche Forschungsgemeinschaft (DFG) Emmy Noether Research Group DA 1657/2-1 and GRK 1789 (CEMMA), funds from the Junior Professorship Program Baden-Wuerttemberg , as well as FWF SFB F-44 and DFG LI-1745/1 . ",
year = "2019",
month = nov,
day = "26",
doi = "10.1016/j.celrep.2019.10.089",
language = "English (US)",
volume = "29",
pages = "2862--2874.e9",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",
}