In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species

Martin Kiechle; Bjoern von Einem; Lennart Höfs; Patrizia Voehringer; Veselin Grozdanov; Daniel Markx; Rosanna Parlato; Diana Wiesner; Benjamin Mayer; Olena Sakk; Bernd Baumann; Soeren Lukassen; Birgit Liss; Arif B. Ekici; Albert C. Ludolph; Paul Walther; Boris Ferger; Pamela J. McLean; Björn H. Falkenburger; Jochen H. Weishaupt; Karin M. Danzer

doi:10.1016/j.celrep.2019.10.089

In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species

Martin Kiechle, Bjoern von Einem, Lennart Höfs, Patrizia Voehringer, Veselin Grozdanov, Daniel Markx, Rosanna Parlato, Diana Wiesner, Benjamin Mayer, Olena Sakk, Bernd Baumann, Soeren Lukassen, Birgit Liss, Arif B. Ekici, Albert C. Ludolph, Paul Walther, Boris Ferger, Pamela J. McLean, Björn H. Falkenburger, Jochen H. WeishauptKarin M. Danzer

Neuroscience

Research output: Contribution to journal › Article

Abstract

Kiechle et al. present two transgenic mouse models that allow direct quantitative and spatial detection of α-synuclein (α-syn) oligomers in vivo. They demonstrate α-syn aggregation at the synapse, age-dependent accumulation of 8–16-mer α-syn oligomer species, and trans-cellular oligomer spreading from forebrain to hindbrain neurons.

Original language	English (US)
Pages (from-to)	2862-2874.e9
Journal	Cell reports
Volume	29
Issue number	9
DOIs	https://doi.org/10.1016/j.celrep.2019.10.089
State	Published - Nov 26 2019

Keywords

aging
alpha-synuclein
neurodegeneration
oligomerization
Parkinson's disease
progressive phenotype
protein-fragment complementation
spreading
synaptic oligomers
transgenic mouse model

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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Kiechle, M., von Einem, B., Höfs, L., Voehringer, P., Grozdanov, V., Markx, D., Parlato, R., Wiesner, D., Mayer, B., Sakk, O., Baumann, B., Lukassen, S., Liss, B., Ekici, A. B., Ludolph, A. C., Walther, P., Ferger, B., McLean, P. J., Falkenburger, B. H., ... Danzer, K. M. (2019). In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species. Cell reports, 29(9), 2862-2874.e9. https://doi.org/10.1016/j.celrep.2019.10.089

In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species. / Kiechle, Martin; von Einem, Bjoern; Höfs, Lennart; Voehringer, Patrizia; Grozdanov, Veselin; Markx, Daniel; Parlato, Rosanna; Wiesner, Diana; Mayer, Benjamin; Sakk, Olena; Baumann, Bernd; Lukassen, Soeren; Liss, Birgit; Ekici, Arif B.; Ludolph, Albert C.; Walther, Paul; Ferger, Boris; McLean, Pamela J.; Falkenburger, Björn H.; Weishaupt, Jochen H.; Danzer, Karin M.

In: Cell reports, Vol. 29, No. 9, 26.11.2019, p. 2862-2874.e9.

Research output: Contribution to journal › Article

Kiechle, M, von Einem, B, Höfs, L, Voehringer, P, Grozdanov, V, Markx, D, Parlato, R, Wiesner, D, Mayer, B, Sakk, O, Baumann, B, Lukassen, S, Liss, B, Ekici, AB, Ludolph, AC, Walther, P, Ferger, B, McLean, PJ, Falkenburger, BH, Weishaupt, JH & Danzer, KM 2019, 'In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species', Cell reports, vol. 29, no. 9, pp. 2862-2874.e9. https://doi.org/10.1016/j.celrep.2019.10.089

Kiechle, Martin ; von Einem, Bjoern ; Höfs, Lennart ; Voehringer, Patrizia ; Grozdanov, Veselin ; Markx, Daniel ; Parlato, Rosanna ; Wiesner, Diana ; Mayer, Benjamin ; Sakk, Olena ; Baumann, Bernd ; Lukassen, Soeren ; Liss, Birgit ; Ekici, Arif B. ; Ludolph, Albert C. ; Walther, Paul ; Ferger, Boris ; McLean, Pamela J. ; Falkenburger, Björn H. ; Weishaupt, Jochen H. ; Danzer, Karin M. / In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species. In: Cell reports. 2019 ; Vol. 29, No. 9. pp. 2862-2874.e9.

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abstract = "Kiechle et al. present two transgenic mouse models that allow direct quantitative and spatial detection of α-synuclein (α-syn) oligomers in vivo. They demonstrate α-syn aggregation at the synapse, age-dependent accumulation of 8–16-mer α-syn oligomer species, and trans-cellular oligomer spreading from forebrain to hindbrain neurons.",

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AU - Voehringer, Patrizia

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AU - Parlato, Rosanna

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AU - Baumann, Bernd

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