In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species

Martin Kiechle, Bjoern von Einem, Lennart Höfs, Patrizia Voehringer, Veselin Grozdanov, Daniel Markx, Rosanna Parlato, Diana Wiesner, Benjamin Mayer, Olena Sakk, Bernd Baumann, Soeren Lukassen, Birgit Liss, Arif B. Ekici, Albert C. Ludolph, Paul Walther, Boris Ferger, Pamela J. McLean, Björn H. Falkenburger, Jochen H. WeishauptKarin M. Danzer

Research output: Contribution to journal › Article

Abstract

Kiechle et al. present two transgenic mouse models that allow direct quantitative and spatial detection of α-synuclein (α-syn) oligomers in vivo. They demonstrate α-syn aggregation at the synapse, age-dependent accumulation of 8–16-mer α-syn oligomer species, and trans-cellular oligomer spreading from forebrain to hindbrain neurons.

Original languageEnglish (US)
Pages (from-to)2862-2874.e9
JournalCell reports
Volume29
Issue number9
DOIs
StatePublished - Nov 26 2019

Fingerprint

Synucleins
Oligomerization
Rhombencephalon
Prosencephalon
Oligomers
Synapses
Transgenic Mice
Neurons
Proteins
Agglomeration

Keywords

  • aging
  • alpha-synuclein
  • neurodegeneration
  • oligomerization
  • Parkinson's disease
  • progressive phenotype
  • protein-fragment complementation
  • spreading
  • synaptic oligomers
  • transgenic mouse model

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species. / Kiechle, Martin; von Einem, Bjoern; Höfs, Lennart; Voehringer, Patrizia; Grozdanov, Veselin; Markx, Daniel; Parlato, Rosanna; Wiesner, Diana; Mayer, Benjamin; Sakk, Olena; Baumann, Bernd; Lukassen, Soeren; Liss, Birgit; Ekici, Arif B.; Ludolph, Albert C.; Walther, Paul; Ferger, Boris; McLean, Pamela J.; Falkenburger, Björn H.; Weishaupt, Jochen H.; Danzer, Karin M.

In: Cell reports, Vol. 29, No. 9, 26.11.2019, p. 2862-2874.e9.

Research output: Contribution to journal › Article

Kiechle, M, von Einem, B, Höfs, L, Voehringer, P, Grozdanov, V, Markx, D, Parlato, R, Wiesner, D, Mayer, B, Sakk, O, Baumann, B, Lukassen, S, Liss, B, Ekici, AB, Ludolph, AC, Walther, P, Ferger, B, McLean, PJ, Falkenburger, BH, Weishaupt, JH & Danzer, KM 2019, 'In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species', Cell reports, vol. 29, no. 9, pp. 2862-2874.e9. https://doi.org/10.1016/j.celrep.2019.10.089
Kiechle M, von Einem B, Höfs L, Voehringer P, Grozdanov V, Markx D et al. In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species. Cell reports. 2019 Nov 26;29(9):2862-2874.e9. https://doi.org/10.1016/j.celrep.2019.10.089
Kiechle, Martin ; von Einem, Bjoern ; Höfs, Lennart ; Voehringer, Patrizia ; Grozdanov, Veselin ; Markx, Daniel ; Parlato, Rosanna ; Wiesner, Diana ; Mayer, Benjamin ; Sakk, Olena ; Baumann, Bernd ; Lukassen, Soeren ; Liss, Birgit ; Ekici, Arif B. ; Ludolph, Albert C. ; Walther, Paul ; Ferger, Boris ; McLean, Pamela J. ; Falkenburger, Björn H. ; Weishaupt, Jochen H. ; Danzer, Karin M. / In Vivo Protein Complementation Demonstrates Presynaptic α-Synuclein Oligomerization and Age-Dependent Accumulation of 8–16-mer Oligomer Species. In: Cell reports. 2019 ; Vol. 29, No. 9. pp. 2862-2874.e9.
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abstract = "Kiechle et al. present two transgenic mouse models that allow direct quantitative and spatial detection of α-synuclein (α-syn) oligomers in vivo. They demonstrate α-syn aggregation at the synapse, age-dependent accumulation of 8–16-mer α-syn oligomer species, and trans-cellular oligomer spreading from forebrain to hindbrain neurons.",
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AU - Voehringer, Patrizia

AU - Grozdanov, Veselin

AU - Markx, Daniel

AU - Parlato, Rosanna

AU - Wiesner, Diana

AU - Mayer, Benjamin

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AU - Baumann, Bernd

AU - Lukassen, Soeren

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AU - Ferger, Boris

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