In Vivo Imaging of Oncolytic Measles Virus Propagation with Single-Cell Resolution

Iris Kemler, Matthew K. Ennis, Claudia M. Neuhauser, David Dingli

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Recombinant measles viruses (MVs) have oncolytic activity against a variety of human cancers. However, their kinetics of spread within tumors has been unexplored. We established an intravital imaging system using the dorsal skin fold chamber, which allows for serial, non-invasive imaging of tumor cells and replication of a fusogenic and a hypofusogenic MV. Hypofusogenic virus-infected cells were detected at the earliest 3 days post-infection (dpi), with peak infection around 6 dpi. In contrast, the fusogenic virus replicated faster: infected cells were detectable 1 dpi and cells were killed quickly. Infection foci were significantly larger with the fusogenic virus. Both viruses formed syncytia. The spatial relationships between cells have a major influence on the outcome of therapy with oncolytic viruses.

Original languageEnglish (US)
Pages (from-to)68-78
Number of pages11
JournalMolecular Therapy - Oncolytics
Volume12
DOIs
StatePublished - Mar 29 2019

Keywords

  • intravital imaging
  • measles
  • oncolytic
  • population dynamics
  • virotherapy
  • virus dynamics

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Cancer Research
  • Pharmacology (medical)

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