TY - JOUR
T1 - Improved survival of mesenchymal stromal cell after hypoxia preconditioning
T2 - Role of oxidative stress
AU - Peterson, Karen M.
AU - Aly, Abdelrahman
AU - Lerman, Amir
AU - Lerman, Lilach O.
AU - Rodriguez-Porcel, Martin
N1 - Funding Information:
This work was supported in part by National Health Lung and Blood Institute R00 HL088048 , and the Mayo Clinical Scholarship Program , Mayo Clinic, Rochester, Minnesota.
PY - 2011/1/3
Y1 - 2011/1/3
N2 - Aims: To investigate the mechanisms underlying the beneficial effect of hypoxia preconditioning (HPC) on mesenchymal stromal cells (MSCs) and optimize novel non-invasive methods to assess the effect of biological interventions aimed to increased cell survival. Main methods: MSCs from rat femur, with or without HPC, were exposed to hypoxic conditions in cell culture (1% O 2 for 24 h) and cell survival (by the LDH release assay and Annexin-V staining) was measured. Oxidant status (conversion of dichloro-fluorescein-DCF- and dihydro-ethidium-DHE-, protein expression of oxidant enzymes) was characterized, together with the mobility pattern of cells under stress. Furthermore, cell survival was assessed non-invasively using state-of-the-art molecular imaging. Key findings: Compared to controls, Hypoxia resulted in increased expression of the oxidative stress enzyme NAD(P)H oxidase (subunit 67phox: 0.05 ± 0.01 AU and 0.48 ± 0.02 AU, respectively, p < 0.05) and in the amount of ROS (DCF: 13 ± 1 and 42 ± 3 RFU/μg protein, respectively, p < 0.05) which led to a decrease in stem cell viability. Hypoxia preconditioning preserved cell biology, as evidenced by preservation of oxidant status (16 ± 1 RFU/μg protein, p < 0.05 vs. hypoxia), and cell viability. Most importantly, the beneficial effect of HPC can be assessed non-invasively using molecular imaging. Significance: HPC preserves cell viability and function, in part through preservation of oxidant status, and its effects can be assessed using state-of-the-art molecular imaging. Understanding of the mechanisms underlying the fate of stem cells will be critical for the advancement of the field of stem cell therapy.
AB - Aims: To investigate the mechanisms underlying the beneficial effect of hypoxia preconditioning (HPC) on mesenchymal stromal cells (MSCs) and optimize novel non-invasive methods to assess the effect of biological interventions aimed to increased cell survival. Main methods: MSCs from rat femur, with or without HPC, were exposed to hypoxic conditions in cell culture (1% O 2 for 24 h) and cell survival (by the LDH release assay and Annexin-V staining) was measured. Oxidant status (conversion of dichloro-fluorescein-DCF- and dihydro-ethidium-DHE-, protein expression of oxidant enzymes) was characterized, together with the mobility pattern of cells under stress. Furthermore, cell survival was assessed non-invasively using state-of-the-art molecular imaging. Key findings: Compared to controls, Hypoxia resulted in increased expression of the oxidative stress enzyme NAD(P)H oxidase (subunit 67phox: 0.05 ± 0.01 AU and 0.48 ± 0.02 AU, respectively, p < 0.05) and in the amount of ROS (DCF: 13 ± 1 and 42 ± 3 RFU/μg protein, respectively, p < 0.05) which led to a decrease in stem cell viability. Hypoxia preconditioning preserved cell biology, as evidenced by preservation of oxidant status (16 ± 1 RFU/μg protein, p < 0.05 vs. hypoxia), and cell viability. Most importantly, the beneficial effect of HPC can be assessed non-invasively using molecular imaging. Significance: HPC preserves cell viability and function, in part through preservation of oxidant status, and its effects can be assessed using state-of-the-art molecular imaging. Understanding of the mechanisms underlying the fate of stem cells will be critical for the advancement of the field of stem cell therapy.
KW - Firefly luciferase
KW - Hypoxia
KW - Hypoxia preconditioning
KW - Mesenchymal stromal cells
KW - Molecular imaging
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U2 - 10.1016/j.lfs.2010.10.023
DO - 10.1016/j.lfs.2010.10.023
M3 - Article
C2 - 21062632
AN - SCOPUS:78650523545
SN - 0024-3205
VL - 88
SP - 65
EP - 73
JO - Life Sciences
JF - Life Sciences
IS - 1-2
ER -