Importance of extending the use of polymerase chain reaction in the diagnosis of venereal syphilis in a blood transfusion center in Burkina Faso, West Africa

Abibou Simpore, Cyrille Bisseye, Bolni Nagalo, Mahamoudou Sanou, Yacoub Nébié, Laure Stella Ghoma-Linguissi, Honorine Dahourou, Boukary Sawadogo, Florencia Djigma, Siaka Ouattara, Virginio Pietra, Jason Nichol, Jacques Simpore

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: Due to the existence of a variety of types of non-venereal syphilis caused by the related T. pallidum, regular serological testing such as Rapid Plasma Reagin (RPR) and Chemiluminescent Microparticle Immunoassay Technique (CMIA) are often unable to differentiate venereal syphilis from the non- venereal one, hence, the interest in the use of molecular biology testing for a confirmation diagnosis of syphilis caused by Treponema pallidum subspecies pallidum. Objective: The study is designed to assess the effectiveness of PCR testing and serological methods in the diagnosis of Treponema pallidum subsp pallidum among blood donors in Burkina Faso. Methods: The study included 6375 samples of volunteer blood donors from the regional blood transfusion center of Ouagadougou (CRTS/O). Among samples, 183 positive and 59 negative in RPR were analyzed to detect antibodies anti-T. pallidum subsp pallidum with a immunoassay method (CMIA) and were confirmed using the Polymerase Chain Reaction testing. Results: In RPR, we obtained a prevalence rate of 2.9% (183/6375) for treponematosis. From the 183 RPR+ specimen, 108 (59%) were found CMIA+ and 11 (6%) were confirmed PCR+. While the 59 pattern RPR-; 31 (52.5%) were CMIA + including 3 (5.1%) tested PCR+. Seventy-five (75) samples RPR+/CMIA-; 2 (2.7%) were confirmed positive by PCR. All 28 samples RPR-/CMIA- were confirmed negative by PCR. Conclusion: PCR testing confirmed a low distribution of T. pallidum subsp pallidum in comparison to serological methods. Cross-reactions, existence of non-venereal treponemal or immunological scars could account for the discrepancy between the results obtained.

Original languageEnglish (US)
JournalPan African Medical Journal
Volume18
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Reagins
Burkina Faso
Western Africa
Syphilis
Globus Pallidus
Immunoassay
Blood Transfusion
Polymerase Chain Reaction
Treponema pallidum
Blood Donors
Cross Reactions
Cicatrix
Molecular Biology
Volunteers
Antibodies

Keywords

  • CMIA
  • Diagnosis
  • PCR
  • RPR
  • Treponema pallidum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Importance of extending the use of polymerase chain reaction in the diagnosis of venereal syphilis in a blood transfusion center in Burkina Faso, West Africa. / Simpore, Abibou; Bisseye, Cyrille; Nagalo, Bolni; Sanou, Mahamoudou; Nébié, Yacoub; Ghoma-Linguissi, Laure Stella; Dahourou, Honorine; Sawadogo, Boukary; Djigma, Florencia; Ouattara, Siaka; Pietra, Virginio; Nichol, Jason; Simpore, Jacques.

In: Pan African Medical Journal, Vol. 18, 01.01.2014.

Research output: Contribution to journalArticle

Simpore, A, Bisseye, C, Nagalo, B, Sanou, M, Nébié, Y, Ghoma-Linguissi, LS, Dahourou, H, Sawadogo, B, Djigma, F, Ouattara, S, Pietra, V, Nichol, J & Simpore, J 2014, 'Importance of extending the use of polymerase chain reaction in the diagnosis of venereal syphilis in a blood transfusion center in Burkina Faso, West Africa', Pan African Medical Journal, vol. 18. https://doi.org/10.11604/pamj.2014.18.56.3850
Simpore, Abibou ; Bisseye, Cyrille ; Nagalo, Bolni ; Sanou, Mahamoudou ; Nébié, Yacoub ; Ghoma-Linguissi, Laure Stella ; Dahourou, Honorine ; Sawadogo, Boukary ; Djigma, Florencia ; Ouattara, Siaka ; Pietra, Virginio ; Nichol, Jason ; Simpore, Jacques. / Importance of extending the use of polymerase chain reaction in the diagnosis of venereal syphilis in a blood transfusion center in Burkina Faso, West Africa. In: Pan African Medical Journal. 2014 ; Vol. 18.
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abstract = "Introduction: Due to the existence of a variety of types of non-venereal syphilis caused by the related T. pallidum, regular serological testing such as Rapid Plasma Reagin (RPR) and Chemiluminescent Microparticle Immunoassay Technique (CMIA) are often unable to differentiate venereal syphilis from the non- venereal one, hence, the interest in the use of molecular biology testing for a confirmation diagnosis of syphilis caused by Treponema pallidum subspecies pallidum. Objective: The study is designed to assess the effectiveness of PCR testing and serological methods in the diagnosis of Treponema pallidum subsp pallidum among blood donors in Burkina Faso. Methods: The study included 6375 samples of volunteer blood donors from the regional blood transfusion center of Ouagadougou (CRTS/O). Among samples, 183 positive and 59 negative in RPR were analyzed to detect antibodies anti-T. pallidum subsp pallidum with a immunoassay method (CMIA) and were confirmed using the Polymerase Chain Reaction testing. Results: In RPR, we obtained a prevalence rate of 2.9{\%} (183/6375) for treponematosis. From the 183 RPR+ specimen, 108 (59{\%}) were found CMIA+ and 11 (6{\%}) were confirmed PCR+. While the 59 pattern RPR-; 31 (52.5{\%}) were CMIA + including 3 (5.1{\%}) tested PCR+. Seventy-five (75) samples RPR+/CMIA-; 2 (2.7{\%}) were confirmed positive by PCR. All 28 samples RPR-/CMIA- were confirmed negative by PCR. Conclusion: PCR testing confirmed a low distribution of T. pallidum subsp pallidum in comparison to serological methods. Cross-reactions, existence of non-venereal treponemal or immunological scars could account for the discrepancy between the results obtained.",
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AU - Bisseye, Cyrille

AU - Nagalo, Bolni

AU - Sanou, Mahamoudou

AU - Nébié, Yacoub

AU - Ghoma-Linguissi, Laure Stella

AU - Dahourou, Honorine

AU - Sawadogo, Boukary

AU - Djigma, Florencia

AU - Ouattara, Siaka

AU - Pietra, Virginio

AU - Nichol, Jason

AU - Simpore, Jacques

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N2 - Introduction: Due to the existence of a variety of types of non-venereal syphilis caused by the related T. pallidum, regular serological testing such as Rapid Plasma Reagin (RPR) and Chemiluminescent Microparticle Immunoassay Technique (CMIA) are often unable to differentiate venereal syphilis from the non- venereal one, hence, the interest in the use of molecular biology testing for a confirmation diagnosis of syphilis caused by Treponema pallidum subspecies pallidum. Objective: The study is designed to assess the effectiveness of PCR testing and serological methods in the diagnosis of Treponema pallidum subsp pallidum among blood donors in Burkina Faso. Methods: The study included 6375 samples of volunteer blood donors from the regional blood transfusion center of Ouagadougou (CRTS/O). Among samples, 183 positive and 59 negative in RPR were analyzed to detect antibodies anti-T. pallidum subsp pallidum with a immunoassay method (CMIA) and were confirmed using the Polymerase Chain Reaction testing. Results: In RPR, we obtained a prevalence rate of 2.9% (183/6375) for treponematosis. From the 183 RPR+ specimen, 108 (59%) were found CMIA+ and 11 (6%) were confirmed PCR+. While the 59 pattern RPR-; 31 (52.5%) were CMIA + including 3 (5.1%) tested PCR+. Seventy-five (75) samples RPR+/CMIA-; 2 (2.7%) were confirmed positive by PCR. All 28 samples RPR-/CMIA- were confirmed negative by PCR. Conclusion: PCR testing confirmed a low distribution of T. pallidum subsp pallidum in comparison to serological methods. Cross-reactions, existence of non-venereal treponemal or immunological scars could account for the discrepancy between the results obtained.

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