Abstract
To evaluate the impact of uremia and associated caloric restriction on physiologically pulsatile growth hormone (GH) release, we used deconvolution analysis of spontaneous plasma GH profiles in 5/6-nephrectomized male rats (NX, N = 9). Three different normal renal function sham-operated groups were used: rats fed a normal diet ad libitum (SAL, N = 9); NX pair-fed rats (SPF, N = 6); NX rats pair-fed for protein ingestion but calorically supplemented up to the energy intake of SAL (SPF+, N = 8). Severe renal failure was confirmed by much higher (P < 0.001) BUN in NX than sham groups. NX rats were growth retarded as shown by reduced (P < 0.01) weight and length gains as compared with sham animals. Deconvolution analysis (mean ± SEM) of plasma samples obtained every 10 minutes over 6 hours, and 14 to 16 days after second stage nephrectomy showed that NX rats had a longer GH t 1/4 (17.0 ± 1.8 vs. 11.6 ± 0.8 min), less GH mass secreted per burst (48 ± 15 vs. 95 ± 16 ng/ml/pulse), lower secretory pulse amplitude (1.9 ± 0.5 vs. 5.8 ± 0.9 ng/ml/min), and a reduced total GH secretion (240 ± 69 vs. 400 ± 56 ng/ml/6 hr) than SAL rats. Corresponding data were not significantly different between NX and SPF, or between SAL and SPF+groups. In summary, stunted rats with chronic renal failure exhibit a prolonged GH t 1/4 and suppression of GH secretory pattern burst mass. Control data from rats with normal renal function suggest that the amplitude-specific depression of GH secretion may be attributed, at least in part, to chronic renal failure-associated calorie deficiency.
Original language | English (US) |
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Pages (from-to) | 648-653 |
Number of pages | 6 |
Journal | Kidney international |
Volume | 52 |
Issue number | 3 |
DOIs | |
State | Published - 1997 |
Keywords
- Caloric deficiency
- Chronic renal failure
- Diet and growth
- Growth hormone
- Nephrectomy
- Uremia
ASJC Scopus subject areas
- Nephrology