Immunomodulatory role of IL-4 on the secretion of Ig by human B cells

J. B. Splawski, Diane F Jelinek, P. E. Lipsky

Research output: Contribution to journalArticle

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Abstract

The effect of IL-4 on the production of Ig by human B cells was examined. Highly purified B cells were stimulated with Staphylococcus aureus (SA) and IL-4 alone or in combination with various other cytokines and the supernatants assayed for Ig by isotype-specific ELISA. IL-4 (10 to 100 U/ml) did not support Ig secretion by SA-stimulated blood, spleen, or lymph node B cells, whereas IL-2 supported the production of all isotypes including IgE. Moreover, IL-4 suppressed the production of all isotypes of Ig by B cells stimulated with SA and IL-2 including IgG1, IgG2, and IgE. IL-4-mediated suppression was partially reversed by IFN-γ or -α and low m.w. B cell growth factor. TNF-α and IL-6 did not reverse the IL-4-induced suppression of Ig production. The inhibitory action of IL-4 on Ig production appeared to depend on the polyclonal activator used to stimulate the B cells. Thus, Ig secretion by B cells activated by LPS and supported by IL-2 was not inhibited by IL-4. Whereas IL-4 alone supported minimal Ig production by LPS-activated B cells, it augmented production of all Ig isotypes in cultures stimulated with LPS and supported by IL-2. IFN-γ further enhanced production of Ig in these cultures. When the effect of IL-4 on the responsiveness of B cells preactivated with SA and IL-2 was examined, it was found not to inhibit but rather to promote Ig production modestly. A direct effect of IL-4 on the terminal differentiation of B cells was demonstrated using B lymphoblastoid cell lines. IL-4 was able to enhance the Ig secreted by an IgA-secreting hybridoma, 219 and by SKW6-CL-4, an IL-6-responsive IgM-secreting EBV transformed B cell line. These results indicate that IL-4 exerts a number of immunoregulatory actions on human B cell differentiation. It interferes with the activation of B cells by SA and IL-2, but promotes the differentiation of preactivated B cells, B cell lines, and B cells activated by LPS without apparent isotype specificity.

Original languageEnglish (US)
Pages (from-to)1569-1575
Number of pages7
JournalJournal of Immunology
Volume142
Issue number5
StatePublished - 1989
Externally publishedYes

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Interleukin-4
B-Lymphocytes
Interleukin-2
Staphylococcus aureus
Immunoglobulin E
Interleukin-6
Immunoglobulin G
Cell Line
Transformed Cell Line
Hybridomas
Human Herpesvirus 4
Interleukin-10
Immunoglobulin A
Immunoglobulin M
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Immunomodulatory role of IL-4 on the secretion of Ig by human B cells. / Splawski, J. B.; Jelinek, Diane F; Lipsky, P. E.

In: Journal of Immunology, Vol. 142, No. 5, 1989, p. 1569-1575.

Research output: Contribution to journalArticle

Splawski, JB, Jelinek, DF & Lipsky, PE 1989, 'Immunomodulatory role of IL-4 on the secretion of Ig by human B cells', Journal of Immunology, vol. 142, no. 5, pp. 1569-1575.
Splawski, J. B. ; Jelinek, Diane F ; Lipsky, P. E. / Immunomodulatory role of IL-4 on the secretion of Ig by human B cells. In: Journal of Immunology. 1989 ; Vol. 142, No. 5. pp. 1569-1575.
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