Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells

E. F. Plow, Joseph C Loftus, E. G. Levin, D. S. Fair, D. Dixon, J. Forsyth, M. H. Ginsberg

Research output: Contribution to journalArticle

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Abstract

A polyclonal antiserum to platelet membrane glycoprotein GPIIb/IIIa was used to detect antigenically related molecules on a diverse panel of human cells. Umbilical vein endothelial cells, erythroleukemic HEL cell, and diploid fetal lung GM1380 fibroblasts expressed GPIIb/IIIa-related molecules, as judged by immunofluorescence and immunoprecipitation of surface-labeled proteins. The GPIIb and BPIIIa subunits were both present and were of similar molecular weight in these cell types. These molecules were synthetic products of the cells, as shown by immunoprecipitation of intrinsically labeled proteins. Promyeloid U937 cells could be induced by 4β-phorbol 12-myristate 13-acetate to synthetize and express GPIIb/IIIa-related molecules on their cell surface. The GPIIb/IIIa-related molecules were not precisely identical in the various cell types, based on slight differences in electrophoretic mobility and their failure to react with monoclonal antibodies specific for each subunit of platelet GPIIb/IIIa. These results suggest the existence of a widely distributed family of GPIIb/IIIa-related molecules. This family of 'cytoadhesins' may share a common function in cellular adhesive reactions.

Original languageEnglish (US)
Pages (from-to)6002-6006
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number16
DOIs
StatePublished - 1986
Externally publishedYes

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Integrin beta3
Immunoprecipitation
Artificial Cells
U937 Cells
Umbilical Veins
Diploidy
Adhesives
Fluorescent Antibody Technique
Immune Sera
Membrane Proteins
Acetates
Blood Platelets
Endothelial Cells
Fibroblasts
Molecular Weight
Monoclonal Antibodies
Lung
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells. / Plow, E. F.; Loftus, Joseph C; Levin, E. G.; Fair, D. S.; Dixon, D.; Forsyth, J.; Ginsberg, M. H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 16, 1986, p. 6002-6006.

Research output: Contribution to journalArticle

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