Immunofluorescence on pronase-digested paraffin sections: A valuable salvage technique for renal biopsies

S. H. Nasr, S. J. Galgano, G. S. Markowitz, M. B. Stokes, V. D. D'Agati

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Direct immunofluorescence (IF) on frozen tissue is the method of choice for the study of medical renal diseases. When no glomeruli are available, IF can be performed on the formalin-fixed paraffin-embedded tissue allocated for light microscopy after antigen retrieval with proteases. In this study, the results of IF on frozen tissue (IF-F) and on deparaffinized, pronase-treated tissue (IF-P) were compared in 71 renal biopsies representing 12 major renal diseases. Using IF-P, diagnostic findings were obtained in 100% of cases of lupus nephritis, acute post-infectious glomerulonephritis, cryoglobulinemic glomerulonephritis, fibrillary glomerulonephritis, primary amyloidosis, myeloma cast nephropathy, and light-chain Fanconi syndrome (LCFS), 88% of cases of immunoglobulin (Ig)A nephropathy, 80% of cases of light-chain deposition disease, 60% of cases of membranoproliferative glomerulonephritis type 1, 50% of cases of idiopathic membranous glomerulopathy (MGN) and 20% of cases of anti-glomerular basement membrane (GBM) disease. IF-P was less sensitive than IF-F for the detection of C3 in all disease categories and for the detection of IgG in cases of MGN and anti-GBM disease. The diagnostic kappa light-chain staining was demonstrated in 100% of cases of LCFS by IF-P versus 40% by IF-F. We conclude that IF-P is a valuable salvage immunohistochemical technique for renal biopsies lacking adequate cortical sampling for IF-F, and is superior to IF-F for the diagnosis of LCFS.

Original languageEnglish (US)
Pages (from-to)2148-2151
Number of pages4
JournalKidney International
Volume70
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Pronase
Paraffin
Fluorescent Antibody Technique
Kidney
Biopsy
Fanconi Syndrome
Light
Glomerulonephritis
Anti-Glomerular Basement Membrane Disease
IGA Glomerulonephritis
Membranoproliferative Glomerulonephritis
Direct Fluorescent Antibody Technique
Membranous Glomerulonephritis
Lupus Nephritis
Formaldehyde
Microscopy
Peptide Hydrolases
Immunoglobulin G

Keywords

  • Immunofluorescence
  • Paraffin-embedded tissue
  • Pronase

ASJC Scopus subject areas

  • Nephrology

Cite this

Nasr, S. H., Galgano, S. J., Markowitz, G. S., Stokes, M. B., & D'Agati, V. D. (2006). Immunofluorescence on pronase-digested paraffin sections: A valuable salvage technique for renal biopsies. Kidney International, 70(12), 2148-2151. https://doi.org/10.1038/sj.ki.5001990

Immunofluorescence on pronase-digested paraffin sections : A valuable salvage technique for renal biopsies. / Nasr, S. H.; Galgano, S. J.; Markowitz, G. S.; Stokes, M. B.; D'Agati, V. D.

In: Kidney International, Vol. 70, No. 12, 12.2006, p. 2148-2151.

Research output: Contribution to journalArticle

Nasr, S. H. ; Galgano, S. J. ; Markowitz, G. S. ; Stokes, M. B. ; D'Agati, V. D. / Immunofluorescence on pronase-digested paraffin sections : A valuable salvage technique for renal biopsies. In: Kidney International. 2006 ; Vol. 70, No. 12. pp. 2148-2151.
@article{30c08a7a03494c80bca594b5d828aa74,
title = "Immunofluorescence on pronase-digested paraffin sections: A valuable salvage technique for renal biopsies",
abstract = "Direct immunofluorescence (IF) on frozen tissue is the method of choice for the study of medical renal diseases. When no glomeruli are available, IF can be performed on the formalin-fixed paraffin-embedded tissue allocated for light microscopy after antigen retrieval with proteases. In this study, the results of IF on frozen tissue (IF-F) and on deparaffinized, pronase-treated tissue (IF-P) were compared in 71 renal biopsies representing 12 major renal diseases. Using IF-P, diagnostic findings were obtained in 100{\%} of cases of lupus nephritis, acute post-infectious glomerulonephritis, cryoglobulinemic glomerulonephritis, fibrillary glomerulonephritis, primary amyloidosis, myeloma cast nephropathy, and light-chain Fanconi syndrome (LCFS), 88{\%} of cases of immunoglobulin (Ig)A nephropathy, 80{\%} of cases of light-chain deposition disease, 60{\%} of cases of membranoproliferative glomerulonephritis type 1, 50{\%} of cases of idiopathic membranous glomerulopathy (MGN) and 20{\%} of cases of anti-glomerular basement membrane (GBM) disease. IF-P was less sensitive than IF-F for the detection of C3 in all disease categories and for the detection of IgG in cases of MGN and anti-GBM disease. The diagnostic kappa light-chain staining was demonstrated in 100{\%} of cases of LCFS by IF-P versus 40{\%} by IF-F. We conclude that IF-P is a valuable salvage immunohistochemical technique for renal biopsies lacking adequate cortical sampling for IF-F, and is superior to IF-F for the diagnosis of LCFS.",
keywords = "Immunofluorescence, Paraffin-embedded tissue, Pronase",
author = "Nasr, {S. H.} and Galgano, {S. J.} and Markowitz, {G. S.} and Stokes, {M. B.} and D'Agati, {V. D.}",
year = "2006",
month = "12",
doi = "10.1038/sj.ki.5001990",
language = "English (US)",
volume = "70",
pages = "2148--2151",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Immunofluorescence on pronase-digested paraffin sections

T2 - A valuable salvage technique for renal biopsies

AU - Nasr, S. H.

AU - Galgano, S. J.

AU - Markowitz, G. S.

AU - Stokes, M. B.

AU - D'Agati, V. D.

PY - 2006/12

Y1 - 2006/12

N2 - Direct immunofluorescence (IF) on frozen tissue is the method of choice for the study of medical renal diseases. When no glomeruli are available, IF can be performed on the formalin-fixed paraffin-embedded tissue allocated for light microscopy after antigen retrieval with proteases. In this study, the results of IF on frozen tissue (IF-F) and on deparaffinized, pronase-treated tissue (IF-P) were compared in 71 renal biopsies representing 12 major renal diseases. Using IF-P, diagnostic findings were obtained in 100% of cases of lupus nephritis, acute post-infectious glomerulonephritis, cryoglobulinemic glomerulonephritis, fibrillary glomerulonephritis, primary amyloidosis, myeloma cast nephropathy, and light-chain Fanconi syndrome (LCFS), 88% of cases of immunoglobulin (Ig)A nephropathy, 80% of cases of light-chain deposition disease, 60% of cases of membranoproliferative glomerulonephritis type 1, 50% of cases of idiopathic membranous glomerulopathy (MGN) and 20% of cases of anti-glomerular basement membrane (GBM) disease. IF-P was less sensitive than IF-F for the detection of C3 in all disease categories and for the detection of IgG in cases of MGN and anti-GBM disease. The diagnostic kappa light-chain staining was demonstrated in 100% of cases of LCFS by IF-P versus 40% by IF-F. We conclude that IF-P is a valuable salvage immunohistochemical technique for renal biopsies lacking adequate cortical sampling for IF-F, and is superior to IF-F for the diagnosis of LCFS.

AB - Direct immunofluorescence (IF) on frozen tissue is the method of choice for the study of medical renal diseases. When no glomeruli are available, IF can be performed on the formalin-fixed paraffin-embedded tissue allocated for light microscopy after antigen retrieval with proteases. In this study, the results of IF on frozen tissue (IF-F) and on deparaffinized, pronase-treated tissue (IF-P) were compared in 71 renal biopsies representing 12 major renal diseases. Using IF-P, diagnostic findings were obtained in 100% of cases of lupus nephritis, acute post-infectious glomerulonephritis, cryoglobulinemic glomerulonephritis, fibrillary glomerulonephritis, primary amyloidosis, myeloma cast nephropathy, and light-chain Fanconi syndrome (LCFS), 88% of cases of immunoglobulin (Ig)A nephropathy, 80% of cases of light-chain deposition disease, 60% of cases of membranoproliferative glomerulonephritis type 1, 50% of cases of idiopathic membranous glomerulopathy (MGN) and 20% of cases of anti-glomerular basement membrane (GBM) disease. IF-P was less sensitive than IF-F for the detection of C3 in all disease categories and for the detection of IgG in cases of MGN and anti-GBM disease. The diagnostic kappa light-chain staining was demonstrated in 100% of cases of LCFS by IF-P versus 40% by IF-F. We conclude that IF-P is a valuable salvage immunohistochemical technique for renal biopsies lacking adequate cortical sampling for IF-F, and is superior to IF-F for the diagnosis of LCFS.

KW - Immunofluorescence

KW - Paraffin-embedded tissue

KW - Pronase

UR - http://www.scopus.com/inward/record.url?scp=33845291158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845291158&partnerID=8YFLogxK

U2 - 10.1038/sj.ki.5001990

DO - 10.1038/sj.ki.5001990

M3 - Article

C2 - 17063172

AN - SCOPUS:33845291158

VL - 70

SP - 2148

EP - 2151

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 12

ER -