Immunochemistry of mammalian cholinesterases

William Stephen Brimijoin, Z. Rakonczay, K. Mintz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Advances in the study of cholinesterase biology have been facilitated by the development of polyclonal and monoclonal antibodies to acetylcholinesterase (AChE) (EC 3.1.1.7) and butyrylcholinesterase (BuChE) (EC 3.1.1.8) in several laboratories. Our work has focused on murine monoclonal antibodies to the mammalian enzymes. Two dozen antibodies are now in hand, with primary specificity for the AChE of human red blood cells, rabbit brain, and rat brain, and for the BuChE of human plasma. These antibodies exhibit a restricted but useful range of affinities for other mammalian cholinesterases of corresponding types. Several applications are described, including an analysis of BuChE phylogeny within the higher primates, an immunodisplacement assay of AChE in normal human red blood cells and cells from patients with paroxysmal nocturnal hemoglobinuria, a study of immunochemical differences between membrane-associated and soluble AChE of rabbit brain, and initial work on the immunofluorescence cytochemistry of the rat brain.

Original languageEnglish (US)
Pages (from-to)2960-2964
Number of pages5
JournalFederation Proceedings
Volume45
Issue number13
StatePublished - 1986

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Immunochemistry
Cholinesterases
Acetylcholinesterase
Butyrylcholinesterase
Brain
Erythrocytes
Monoclonal Antibodies
Rabbits
Paroxysmal Hemoglobinuria
Histocytochemistry
Antibodies
Phylogeny
Primates
Fluorescent Antibody Technique
Hand
Membranes
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Brimijoin, W. S., Rakonczay, Z., & Mintz, K. (1986). Immunochemistry of mammalian cholinesterases. Federation Proceedings, 45(13), 2960-2964.

Immunochemistry of mammalian cholinesterases. / Brimijoin, William Stephen; Rakonczay, Z.; Mintz, K.

In: Federation Proceedings, Vol. 45, No. 13, 1986, p. 2960-2964.

Research output: Contribution to journalArticle

Brimijoin, WS, Rakonczay, Z & Mintz, K 1986, 'Immunochemistry of mammalian cholinesterases', Federation Proceedings, vol. 45, no. 13, pp. 2960-2964.
Brimijoin, William Stephen ; Rakonczay, Z. ; Mintz, K. / Immunochemistry of mammalian cholinesterases. In: Federation Proceedings. 1986 ; Vol. 45, No. 13. pp. 2960-2964.
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