TY - JOUR
T1 - Immune cell populations differ in patients undergoing revision total knee arthroplasty for arthrofibrosis
AU - Limberg, Afton K.
AU - Salib, Christopher G.
AU - Tibbo, Meagan E.
AU - Vargas-Hernandez, Juan S.
AU - Bettencourt, Jacob W.
AU - Bayram, Banu
AU - Berry, Charlotte E.
AU - Dudakovic, Amel
AU - Bolon, Brad
AU - van Wijnen, Andre J
AU - Morrey, Mark E.
AU - Sanchez-Sotelo, Joaquin
AU - Berry, Daniel J.
AU - Carter, Jodi
AU - Abdel, Matthew P.
N1 - Funding Information:
Research reported in this publication was supported by Anna-Maria and Stephen Kellen Foundation and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number AR072597 (MPA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the U.S. National Institutes of Health. We would like to thank the entire Mayo Clinic Pathology Research Core Facility and the Mayo Clinic Department of Anatomic Pathology for their expertise in immunohistochemistry, specifically Vivian Negron and Trynda N. Kroneman, CT (ASCP), SCT (ASCP) for their expertise in data processing and collection throughout the duration of this study.
Funding Information:
Research reported in this publication was supported by Anna-Maria and Stephen Kellen Foundation and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number AR072597 (MPA). The content is solely the responsibility of the authors and does not necessarily represent the official views of the U.S. National Institutes of Health. We would like to thank the entire Mayo Clinic Pathology Research Core Facility and the Mayo Clinic Department of Anatomic Pathology for their expertise in immunohistochemistry, specifically Vivian Negron and Trynda N. Kroneman, CT (ASCP), SCT (ASCP) for their expertise in data processing and collection throughout the duration of this study.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Arthrofibrosis following total knee arthroplasty (TKA) is a debilitating condition typically diagnosed based on clinical findings. To gain insight into the histopathologic immune cell microenvironment of arthrofibrosis, we assessed the extent of tissue fibrosis and quantified immune cell populations in specific tissue regions of the posterior capsule. We investigated specimens from three prospectively-collected, matched cohorts, grouped as patients receiving a primary TKA for osteoarthritis, revision TKA for arthrofibrosis, and revision TKA for non-arthrofibrotic, non-infectious reasons. Specimens were evaluated using hematoxylin and eosin staining, picrosirius red staining, immunofluorescence, and immunohistochemistry with Aperio®-based digital image analysis. Increased collagen deposition and increased number of α-SMA/ACTA2 expressing myofibroblasts were present in the arthrofibrosis group compared to the two non-arthrofibrotic groups. CD163 + macrophages were the most abundant immune cell type in any capsular sample with specific enrichment in the synovial tissue. CD163 + macrophages were significantly decreased in the fibrotic tissue region of arthrofibrosis patients compared to the patients with primary TKA, and significantly increased in adipose tissue region of arthrofibrotic specimens compared to non-arthrofibrotic specimens. Synovial CD117 + mast cells were significantly decreased in arthrofibrotic adipose tissue. Together, these findings inform diagnostic and targeted therapeutic strategies by providing insight into the underlying pathogenetic mechanisms of arthrofibrosis.
AB - Arthrofibrosis following total knee arthroplasty (TKA) is a debilitating condition typically diagnosed based on clinical findings. To gain insight into the histopathologic immune cell microenvironment of arthrofibrosis, we assessed the extent of tissue fibrosis and quantified immune cell populations in specific tissue regions of the posterior capsule. We investigated specimens from three prospectively-collected, matched cohorts, grouped as patients receiving a primary TKA for osteoarthritis, revision TKA for arthrofibrosis, and revision TKA for non-arthrofibrotic, non-infectious reasons. Specimens were evaluated using hematoxylin and eosin staining, picrosirius red staining, immunofluorescence, and immunohistochemistry with Aperio®-based digital image analysis. Increased collagen deposition and increased number of α-SMA/ACTA2 expressing myofibroblasts were present in the arthrofibrosis group compared to the two non-arthrofibrotic groups. CD163 + macrophages were the most abundant immune cell type in any capsular sample with specific enrichment in the synovial tissue. CD163 + macrophages were significantly decreased in the fibrotic tissue region of arthrofibrosis patients compared to the patients with primary TKA, and significantly increased in adipose tissue region of arthrofibrotic specimens compared to non-arthrofibrotic specimens. Synovial CD117 + mast cells were significantly decreased in arthrofibrotic adipose tissue. Together, these findings inform diagnostic and targeted therapeutic strategies by providing insight into the underlying pathogenetic mechanisms of arthrofibrosis.
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U2 - 10.1038/s41598-022-22175-3
DO - 10.1038/s41598-022-22175-3
M3 - Article
C2 - 36587032
AN - SCOPUS:85145361635
VL - 12
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 22627
ER -